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GA3?Regulation in the Dwarfing Mutant Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年03期
Page:
456-460
Research Field:
Publishing date:

Info

Title:
GA3?Regulation in the Dwarfing Mutant Soybean
Author(s):
ZHANG DaWANG Jun-hongWANG Yu-yingCANG JingHAO Zai-bin
Life Science College of Northeast Agricultural University,Harbin 150030,Heilongjiang,China
Keywords:
SoybeanDwarf mutantGA3ELISA
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0456
Abstract:
Dwarf,an excellent agronomic character,was thought to play important roles in improving the growing density and enhancing the photosynthesis efficiency.The dwarf mutant Dongze 11 was obtained from Dongnong 42 induced by chemical NaN3.Through the exogenous GA3application and endogenesis GA3content measurement,Dongze 11 and Dongnong 42 were used as research materials to study into how GA3phytohormone worked in the dwarf mutant.The results indicated that the exogenous GA3could apparently promote the stem elongation of the dwarf mutant,and this effect became more significant with treating time accumulated.Among different concentrations of GA3,0.6 mmol.L-1GA3 performed especially well.The application of 2.9×10-4mmol.L-1GA3 had obvious effect on elongation of young stem in vivo,implying that the endogenesis GA3content in mutant was very low,and in this assay,the treatment of 12 hours was most effective.Endogenesis GA3contents during different growth stage was determined by ELISA(Enzyme-linked immunosorbent assay),and results showed that the mutant had lower GA3level than the wild plant,suggesting the shortened stem in mutant should related with the lower GA3content.

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Last Update: 2014-10-08