|Table of Contents|

Tagging SSR Markers for Resistant Gene to Phytophthora Root Rot in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年03期
Page:
379-382
Research Field:
Publishing date:

Info

Title:
Tagging SSR Markers for Resistant Gene to Phytophthora Root Rot in Soybean
Author(s):
LIU Li-jun; SUN Xin; XUE Yong-guo; YANG Zhe
Soybean Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
Keywords:
Soybean Phytophthora root rot;  SSR; BSA
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0379
Abstract:
Phytophthora root rot is an important soybean disease，which can influence the yield.To map SSR makers link with resistant genes to soybean Phytophthora root rot，Hefeng 25 and Kangxian 2 were crossed and the F2 seperation population was obtained.350 pair of SSR primers were screened and 52 pairs have polymorphism.BSA(Bulked segregation analysis) method was used to screen the polymorphic and diverge primers，one pair of polymorphic primer，Scaa003，located on LG J was tagged.The examination rate of Scaa003 between 10 sensitive soybean cultivars and 4 resistant soybean cultivars reached 100%.Result shows that the primer has the common characteristic for the examination，it can provide the theoretic base for selecting the soybean materials which have the resistance to the soybean Phytophthora sojae Race 1.

References:

[1]沈崇尧，苏彦纯.中国大豆疫霉病菌的发现及初步研究[J].植物病理学报，1991，21（4）：289.（Shen C R，Su Y C.Initial research and discovery for Phytophthora sojae in china[J].Acta Phytopathologica Sinica，1991，21（4）：289.）

[2]苏彦纯，沈崇尧．大豆疫霉病菌在中国的发现及其生物学特性的研究[J]．植物病理学报，1993，23（4）：341-347．（Su Y C，Shen C R.Research and discovery of biological characters for Phytophthora sojae[J].Acta Phytopathologica Sinica，1993，23（4）：341-347.）
[3]徐永华，何志鸿.大豆疫霉病早熟抗源[J].大豆通报，1999(3)：23-25.(Xu Y H，He Z H.Early mature resistant materials for soybean Phytophthora Root Rot[J].Soybean Bulletin，1999(3)：23-25.)
[4]霍云龙，朱振东，李向华，等．抗大豆疫霉根腐病野生大豆资源的初步筛[J]．植物遗传资源学报，2005，6（2）：182-185．（Huo Y L，Zhu Z D，Li X H，et al.Preliminary screening for phytophthora root rot resistance in wild soybean[J].Journal of Plant Genetic Resources，2005，6(2)：182-185.）
[5]刘柱，胡新文．分子标记及其在遗传育种中的应用[J]．华南热带农业大学学报，2001，7（1）：21-25.(Liu Z，Hu X W.Application in genetic breeding by using molecular markers[J].Journal of South China University of Tropical Agriculture，2001，7(1)：21-25.)
[6]Cregan P B，Jarvik T，Bush A L，et al.An integrated genetic linkage map of the soybean genome[J].Crop Science，1999，35(5)：1464-1490.
[7]Jee H J，Kim W G，Cho W D.Occurrence of Phytophthora root rot on soybean and identification of the causal fungal[J]．RAD Journal of Crop Protection，1998，40（1）：16-22.
[8]关荣霞，常汝镇，邱丽娟.用于SSR分析的大豆DNA的快速提取[J].大豆科学，2003，21(1)：73-74.(Guan R X，Chang R Z，Qiu L J.Rapid isolation of soybean DNA for SSR analysis[J].Soybean Science，2003，21(1)：73-74.)
[9]Rogers O S，Bendich A J.Extraction of DNA from milligram amounts of fresh，herbarium and mommified plant tissues[J].Plant Molecular Biology，1985，5(2)：69-76.
[10]宛煜嵩.大豆遗传图谱的构建及若干农艺性状的QTL定位分析[D].北京： 中国农业科学院，2002.（Wan Y S.Construction of soybean genetic map and QTL analysis of some agronomic traits[D].Beijing：Chinese Academy of Agricultural Sciences，2002.）
[11]Byrum J R，Kimbirauskas P M，Shoemaker R C.Identification of a RAPD marker introductions and North American ancestors using RAPD and SSR markers[J].Soybean Genetic Newsletter，1993，20： 112-117.
[12]Lohnes D G，Schmitthenner A F．Position of the Phytophthora resistance gene Rps7 on the soybean molecular map[J]．Crop Science，1997，37：555-556．
[13]Kilen T C，Hartwig E E，Keeling B L.Inheritance of a second major gene for resistance to Phytophthora root rot in soybeans[J]．Crop Science，1974，14：260-262．
[14]Buzzell R I，Anderson T R.Another major gene for resistance to Phytophthora introductions and North American ancestors using RAPD and SSR markers[J].Plant Disease，1981，66： 1146-1149.
[15]Weng C，Yu K，Anderson T R，et al.Mapping Genes Conferring Resistance to Phytophthora Root Rot of Soybean，Rps1a andRps7[J].The Journal of Heredity，2001，92(5)：442-446.
[16]Diers B W，Skorupska H T，Rao-Arelli A P，et al． Genetic relationships among soybean plant introductions with resistance to soybean cyst nematodes[J]．Crop Science，1997，37：1966-1972．

Memo

Memo:
-
Last Update: 2014-10-07