|Table of Contents|

High Frequency Plant Regeneration from Whole Cotyledonary Node Explants and Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean [Glycine max(L.)Merrill](PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年03期
Page:
373-378
Research Field:
Publishing date:

Info

Title:
High Frequency Plant Regeneration from Whole Cotyledonary Node Explants and Comparison with Cotyledonary Node and Embryonic Tip Regeneration System in Soybean [Glycine max(L.)Merrill]
Author(s):
MA Xiao-hong;  YAO Lu-ming;  WU Tian-long
School of Agriculture and Biology，Shanghai Jiao Tong University，Shanghai 200240，China
Keywords:
Soybean; Regeneration system; In vitro culture
PACS:
S565.101
DOI:
10.11861/j.issn.1000-9841.2008.03.0373
Abstract:
Soybean [Glycine max(L.)Merrill] in vitro regeneration had been proved to be difficult，which limited the application of gene engineering on soybean.A more efficient and successful regeneration system for soybean was reported here using whole cotyledonary node as the explant.Whole cotyledonary node explants were obtained from aseptic seedlings cultured on MSB5 medium supplemented with N6-benzyladenine(BA)at the concentration of 0.4 mg.L-1for 5-7 d.MSB5 medium containing different concentration and combinations of BA or N-(2-chloro-4-pyridyl)-N’-phenylurea(CPPU)with indole-3-butyric acid(IBA)were selected for shoot regeneration in whole cotyledonary node regeneration system and MSB5 medium containing 3.0 mg.L-1BA and 0.2 mg.L-1 IBA was proved to be optimum.The regenerated shoots were elongated on the MSB5 medium to about 3 cm and rooted on MSB5medium containing IBA 0.5 mg.L-1.Plantlets with well-developed roots were hardened and planted in greenhouse to maturity.Using of this system，shoot regeneration frequency could reach 94.7% and one explant could regenerate 28 shoots on average.In comparison with traditional cotyledonary node and embryonic tip regeneration system，whole cotyledonary node regeneration system was superior in shoot regeneration frequency and number of shoot.

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Last Update: 2014-10-07