|Table of Contents|

An Improved Method For Determining SBA Content and Screening for Soybean Germplasms(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
310-314
Research Field:
Publishing date:

Info

Title:
An Improved Method For Determining SBA Content and Screening for Soybean Germplasms
Author(s):
YANG Ming-liang; SONG Wen-wen; KANG Ming; YANG Zhi-chao; ZHANG Ying; MA Jian; WANG Ji-an
Key Laboratory of Soybean Biology in Chinese Ministry of Education，Northeast Agricultural University，Harbin 150030,Heilongjiang,China
Keywords:
Soybean; SBA content; Determination; Germplasm
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0310
Abstract:
Soybean agglutinin（SBA)，a major anti-nutritional factor，which inhibited the growth of animals，is assessed as an important indicator of animal feed quality.Effective method for determining soybean SBA content will faciliate the screening of soybean germplasms in large scale.In this study，Hemagglutination method of SBA was improved，with which 64 soybean germplasm from soybean Institute of Northeast Agricultural University was analyzed.Results showed the improved method for determining soybean SBA was screening crushed soybean seed with 40 mesh screens，then extracting sample for 10 h with petroleum ether（W∶V) 1∶10 at 60-90℃，and then reacting at pH 5.0-9.0 and at 25℃.In the 64 soybean accessions，the lowest，the highest，and the average content of lectin was respectively 1.88 mgg-1，37.5 mgg-1and 11.91 mgg-1.The improved method has many advantages such as economical，practical，and suitable for screening soybean germplasm in large-scale.

References:

[1]冯琳，周小秋.大豆凝集素（SBA）对动物的抗营养作用[J].饲料工业，2006，27（1）：19-24.(Feng L，Zhou X Q.Soybean lectin（SBA) on the anti-animal nutrition role[J].Feed Industry，2006，27（1）：19-24.)

[2]吴道银.大豆中抗营养因子简介[J].四川粮油科技，2003.（1）：50-51.(Wu D.Y.，Anti-nutritional factors in the soybean introduction[J].Sichuan Cereals Oils and Technology，2003，（1）：50-51.)
[3]丁安林，王燕，常汝镇.大豆抗营养因子及其改良[J].大豆科学， 1994，13（1）：72-76.(Ding A L，Wang Y，Chang R Z.Soybean anti-nutritional factors and improved[J].Soybean Science，1994，13（1）：72-76.)
[4]Goldstein I E，Poretz R D.Isolation，physicochemical characterizationa and carbohydrate binding specificity of lectins［M］∥Liener I E，Sharon N，Goldstein I J.The Lectins：properties，functions and applications in biology and medicine.New York:Academic Press，1986：33-47.
[5]Pusztai A，Watt W B，Stewart J C.A comprehensive scheme for the isolation of trypsin inhibitors and the agglutinin from soybean seeds[J].Journal of Agricultural and Food Chemistry，1991，39：862-866.
[6]Hajos G，Gelencser E，Pusztai A，et al.Biological effects and survival of trypsin inhibitors and the agglutinin from soybean in the small intestine of the rat[J].Journal of Agricultural and Food Chemistry，1995，43:165-170.
[7]Agundis C, Pereyra A, Zenteno R, et al.Quantification of lectin in freshwater prawn（Macrobrachium rosenbergii) hemolymph by ELISA[J].Comparison Biochemical Physiology，2000,127：165-172.
[8]Gueguen J，van Oort M G，Quilien L.Antinutritive factors in peas［M］∥van der Poel AFB，Huisman J，and Saini H S.Recent advances of research inantinutrit-ional factors in legume seeds.Wageningen:Wageningen Perss，1993:9-30.
[9]孙册，朱政，莫汉庆.凝集素[M].北京：科学出版社，1986：20-21.(Sun C，Zhu Z，Mo H Q.Lectin[M].Beijing:Science Press，1986:20-21.)
[10]戴大章，陈妙月，叶均安，等.血凝法测定饲料中植物凝集素含量[J].中国兽医学报，2005,25（4）：438-440.(Dai D Z，Chen M Y，Ye J A，et al.Quantification of lectins in feed by hemagglutination[J].Chinese Journal of Veterinary Science，2005,25（4）：438-440.)

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Last Update: 2014-10-10