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Rapid Approach of Soybean Lipoxygenase’antigen Purification(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
306-309
Research Field:
Publishing date:

Info

Title:
Rapid Approach of Soybean Lipoxygenase’antigen Purification
Author(s):
MA Zhi-min1; 2; 3; LIU Yuan1; 3; JIANG Chun-zhi1; 2; YANG Chun-yan1; 2; MA Zhi-ying1; 3; ZHANG Meng-chen1; 2
1 National Soybean Improvement Center Shijiazhuang Sub-Center，Shijiazhuang 050031,Hebei;
2 Institute of Cereal and Oil Crops，Hebei Academy of Agricultural And Forestry Sciences，Shijiazhuang 050031，Hebei;
3 Agriculture University of Hebei，Baoding 071001，Hebei，China
Keywords:
Lipoxygenase;  Native PAGE;  Electro elution;  Purification
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.02.0306
Abstract:
The common method of purifying protein has the shortcoming of complex operation，time- consuming of purifying，high costing and so on.The objective of this paper is to explore a fast and economical process of protein purification，and the purified protein can be applied to prepare anti-this protein monoclonal antibody.Combining both Native PAGE of the soybean Lipoxygenase and Electro Elution，we purified the lipoxygense from the soybean Jidou No.12 and the Su-1,3，Su-2,2.Lipoxygenase solution is retrieved with electrophoresis degree and recovery rate 49.64% as same of the ion exchange column chromatography.This built a fast and economical approach for antigen purifying，and it is helpful for preparing anti-lipoxygenase monoclonal antibodies and applied to practice even.It also offers important reference to the purification of other protein.

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Last Update: 2014-10-10