|Table of Contents|

Effect of Pathotoxin Produced from Phytophthora sojae on the Ultrastructure of Leaves of Soybean Varieties with Different Resistance(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
288-291
Research Field:
Publishing date:

Info

Title:
Effect of Pathotoxin Produced from Phytophthora sojae on the Ultrastructure of Leaves of Soybean Varieties with Different Resistance
Author(s):
ZHANG Shu-zhen1; XU Peng-fei1; WU Jun-jiang2; LI Wen-bin1; CHEN Wei-yuan3; YU An-liang1;  WANG Jin-sheng1; CHEN Chen1;  JIN Li-mei1; LI Cen1
1Soybean Research Institute，Northeast Agricultural University;Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030，Heilongjiang；
2 Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences（HAAS)，Harbin 150086，Heilongjiang；
3 Suihua Research Institute of HAAS，Suihua 152052，Heilongjiang，China
Keywords:
Phytophthora sojae; Pathotoxin; Varieties with different resistance;  Ultrastructure
PACS:
S435.651
DOI:
10.11861/j.issn.1000-9841.2008.02.0288
Abstract:
Pathotoxin is one of the main pathogenic factors of Phytophthora sojae，which could destroy the cell structure of soybean.To investigate the effect of pathotoxin on cell ultrastructure of soybean，two soybean varieties of Suinong 10(resistant to Phytophthora sojae) and Hefeng 25(susceptible to Phytophthora sojae) were used in this experiment.The excised leaves of the two soybean varieties were dipped into the pathotoxin solution with different concentration（one is the pathotoxin solution of relatively high concentration，0.1794 mgmL-1；the other is pathotoxin solution of optimal concentration，0.0897 mgmL-1），and fixed in the fixation solution after 48 h and 72 h.The ultrastructures of leaves of Suinong 10 and Hefeng 25 were all seriously destroyed after treated with relatively high concentration of pathotoxin solution.The ultrastructure of leaves of resistant soybean Suinong 10 was almost intact after treated with optimal concentration of pathotoxin，but the cell structure of susceptible variety Hefeng 25 changed a lot.The chloroplast grana nearly disappeared，chondriosome appeared vacuolus，and chondriosome envelope disaggregated.The results showed that only optimal concentration of pathotoxin solution could distinguish the difference in cell structure between soybean varieties resistant or susceptible to Phytophthora sojae.

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Last Update: 2014-10-10