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Comparative Studies on Metabolites in White and Brown Cyst of Heterodera Glycines Race 4(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
283-287
Research Field:
Publishing date:

Info

Title:
Comparative Studies on Metabolites in White and Brown Cyst of Heterodera Glycines Race 4
Author(s):
LI Xiu-xia1; WU Hai-yan1; SHI Li-bo1; WANG Zhen-hua1; YU Hao2
1 College of Plant Protection，Shandong Agricultural University，Tai’an 271018，Shandong；
2Academy of Land-reclaimable Sciences of Heilongjiang Province，Jiamusi 154007，Heilongjiang，China
Keywords:
Soybean cyst nematode; Carbohydrate; Esterase; Trehalase; Protein
PACS:
S435.651
DOI:
10.11861/j.issn.1000-9841.2008.02.0283
Abstract:
Previous research stated that the hatch rate of white cysts was significantly different from that of brown cysts，which indicated that there was different diapause rate between white and brown cysts.In order to find out the relationships between some metabolites and diapause of Heterodera glycines，hatch rate and metabolites（total carbohydrate，glycogen，trehalose，glycerol，protein，esterase，trehalase) in white and brown cysts of Heterodera glycines race 4 were studied.The results suggested that hatch rate of brown cyst was lower significantly than that of white cyst.Content of glycogen and protein and activity of esterase in brown cys were lower than that of white cyst，content of total carbohydrate and trehalose and activity of trehalase in brown cyst was higher.Therefore，diapause rate of brown cysts was higher than that of white cysts，and active mutual transformation between trehalose and glycogen existed in brown cysts.SDSPAGE pattern showed that there are 15 and 10 protein bands in white and brown cysts respectively，and molecular weight 116.6，79.1，68.2，64.3 and 60.5 KDa proteins existed in white cysts，and without in brown cysts.There were 3 and 6 bands in patterns of esterase isoenzyme in white and brown cysts respectively，EST-0.63，EST-0.75 and EST-0.81 of esterase isoenzyme occurred in brown cysts，and not in white cysts.The result suggested that content of protein was declined，and activity of esterase isoenzyme was enhanced during the white cyst change to the brown cyst.And deduced that low hatch rate of eggs in brown cysts might be related to those metabolites.

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Last Update: 2014-10-10