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Susceptibility of Soybean Cotyledonary Node to Hygromycin B(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
267-269
Research Field:
Publishing date:

Info

Title:
Susceptibility of Soybean Cotyledonary Node to Hygromycin B
Author(s):
ZOU Li1; 2; WEN Yi2; ZHANG Yun-hua1; WANG Dan2
1Institute of Plant Protection，Heilongjiang Academy of Agricultural Sciences，Harbin 150086;
2 Northeast Forestry University，Harbin 150040，Heilongjiang，China
Keywords:
Soybean; Genotype; Susceptibility; Hygromycin
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0267
Abstract:
The use of maker gene is an efficient method to select and identify transgenic plantlets.Hygromycin can destroy the function of ribosome and restrain plantlets growing.The effects of hygromycin on brown rate，bud differentiation were investigated using 3 soybean varieties.Different mass concentration of hygromycin on the soybean cotyledonary node polarization and bud rooting were studied.The result showed that there was certain difference between the susceptibility of soybean cultivars to selective agents.For cotyledonary node，the optimal mass concentration of hygromycin was 6 mgL-1for Heinong 35，4 mgL-1for Hefeng 35 and Hefeng 25.For rooting，the optimal mass concentration of hygromycin was 2 mgL-1for Heinong 35，1 mgL-1for Hefeng 35 and Hefeng 25.

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Last Update: 2014-10-10