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Reponses of Respiration in Seed Germination of Soybean to Acid Rain Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
255-258,266
Research Field:
Publishing date:

Info

Title:
Reponses of Respiration in Seed Germination of Soybean to Acid Rain Stress
Author(s):
XU Ling-xiang2; DING Shuang2; ZHOU Qing1; 2
1Key Laboratory of Industrial Biotechnology，Ministry of Education，Jiangnan University，Wuxi 214122;
2 School of Environmental and Civil Engineering，Jiangnan University，Wuxi 214122，Jiangsu，China
Keywords:
ean seed; Respiration
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0255
Abstract:
As an important problem affecting agricultural productivity，ecological effect of acid rain on crop seeds germination is highly concerned by researchers at domestic and abroad.Effects of acid rain on soybean germination and respiration were investigated to reveal the mechanism of acid rain on seed germination.Soybean seeds of Shuangqingdou as experimental material，six simulated acid rain（SAR) solutions of pH 2.0，2.5，3.0，3.5，4.0 and 5.0，and neutral solution（pH 6.5) as control group（CK) were used in this study.Pretreated seeds of soybean were placed in culture dishes with filter sheets to germinate in normal temperature，three dishes were involved in each treatment group and 12 seeds were placed in each dish.During germination，seeds were exposed to simulated acid rain that was replaced everyday.The germination indexes（percentage germination，germination energy，germination index)，respiration indexes（respiratory rate（RR)，respiratory quotient（RQ)) and activity of peroxidase(POD)were all studied under acid rain stress.Seed germination was inhibited significantly in high acidity strength（pH 2.0).When pH≥2.5，each solution containing CK had good germination index，but rate of emergence（6 d) was zero and all physiological indexes（1 d) were all abnormal，POD took part in aging.When pH≥3.0，RR decreased slowly with the decreasing pH，and the stress time when RR was significant different from CK was 1 d；RQ decreased as seeds grew to seedlings，and was different from CK；POD activity gradually increased with prolonging stress time and stress strength，and was different from pH 2.5.The threshold value of respiration( pH≥5.0)was the same as that of activity of POD(pH≥5.0)and lower than that of germination（pH≥3.0).Under pH 2.5，3.0，3.5，the stress time when RR（1 d，3 d，3 d)was different from that of CK，and higher than that of POD activity（1 d，1 d，1 d) and RQ（1 d，2 d，2 d).So it could be speculated that stress of acid rain caused stimulation of free radical，deprived electrons on substrates of respiratory chain，and then inhibited respiration；POD，which located in the end of respiratory chain，scavenged free radical and also participated in seed aging.

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Last Update: 2014-10-10