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Expression of Human Granulocyte-Macrophage Colony Stimulating Factor in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
199-202
Research Field:
Publishing date:

Info

Title:
Expression of Human Granulocyte-Macrophage Colony Stimulating Factor in Soybean
Author(s):
LI Xiao-juan; WANG Biao; WU Tian-long
School of Agriculture and Biology，Shanghai Jiao Tong University，Shanghai 200240,China
Keywords:
hGMCSF; Shoot tip; Soybean
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0199
Abstract:
The pCAMBIA3300-CSF-KD containing human granulocyte-macrophage colony stimulating factor（hGM-CSF) was constructed and transformed into soybean. The vector pCAMBIA3300-CSF-KD of hGM-CSF whose terminal has been conjugated，to endoplasmic reticulum retention signal(KDEL)，is fitted for expression in plants. The shoot tip of soybean Hefeng 47 was used as explants to transformation mediated by Agrobacterium tumefaciens，and 17 T1transgenic plants were obtained. Eleven positive plants were verified by PCR amplification.

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Last Update: 2014-10-10