|Table of Contents|

Factors Affecting Particle Bombardment-mediated Transformation of Soybean Germinating Seeds(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
194-198
Research Field:
Publishing date:

Info

Title:
Factors Affecting Particle Bombardment-mediated Transformation of Soybean Germinating Seeds
Author(s):
XUE Ren-gao
College of Life & Science,Qingdao Agricultural University,Qingdao 266109,Shandong,China
Keywords:
Soybean Germinating seed GUS transient expression Particle bombardment
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0194
Abstract:
Although germinating seed of soybean has been successfully used as a target tissue in Agrobacterium-mediated transformation,it has not been investigated in particle bombardment-mediated transformation. In this study,factors such as bombardment times,bombardment distance,culture time,and placing orientation that affect particle bombardment-mediated transformation efficiency were studied via particle bombardment with a plant expression vector pCAMBIA1301 using 1 day-germinating half seeds of soybean as target tissue. The result showed that transformation efficiency based on GUS transient expression was significantly affected by bombardment parameters. The highest GUS transient expression(217.5 blue foci/half seed) was obtained when particle bombardment was carried out under the 2 times of bombardment,9 cm distance of bombardment,and tissue culture for 2 days after bombardment. However,the GUS transient expression was not affected by placing orientation of target tissue.The factors affecting the particle bombardment-mediated transformation of soybean germinating seeds were optimized by GUS transient expression assay. These results will provide useful information for establishment of a particle bombardment-mediated soybean transformation system.

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Last Update: 2014-10-10