|Table of Contents|

Inheritance and Gene Tagging of Male Fertility Restoration of Cytoplasmic-NuclearMale Sterile Line NJCMS2A in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年02期
Page:
181-185
Research Field:
Publishing date:

Info

Title:
Inheritance and Gene Tagging of Male Fertility Restoration of Cytoplasmic-NuclearMale Sterile Line NJCMS2A in Soybean
Author(s):
DONG Jian-sheng; YANG Shou-ping; YU De-yue; GAI Jun-yi
Nanjing Agricultural University，Soybean Research Institute，National Center for Soybean Improvement，National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing 210095，Jiangsu，China
Keywords:
Soybean; Cytoplasmic-nuclear male-sterility; Inheritance; SSR marker
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0181
Abstract:
Cytoplasmic-nuclear male sterility plays an important role in the production of hybrid seeds.The knowledge of inheritance of male fertility restoration of a cytoplasmic-nuclear male-sterile line is essential to hybrid seed production.With the rapid development of molecular markers，mapping male fertility restoration genes appears an effective way to study inheritance of the trait.The soybean cytoplasmic-nuclear male-sterile line NJCMS2A was developed through consecutive backcross procedures with the F2 male sterile plants of the N8855(N1628 cross as donor parent and N1628 (designated as NJCMS2B afterwards) as recurrent parent.In the present paper，the inheritance of the male fertility restoration of NJCMS2A was studied，at the same time，the SSR(simple sequence repeat)marker linked to the restorer gene of NJCMS2A was screened.The results showed that the inheritance of the fertility restoration of NJCMS2A was steady among different years.All F1plants of the NJCMS2A×Zhongdou 5 cross were male-fertile.The F1∶2population exhibited male fertility segregation，and the segretation ratio of male-fertile to male-sterile plants fitted a 15∶1 ratio under χ2 test.The F1and F1∶2results proposed a possible inheritance model of two duplicate dominant genes.The segregation ratio of number of non-segregating male-fertile F2∶3families∶number of 3∶1 segregation F2∶3families∶number of 15∶1 segregation F2∶3families fitted a 7∶4∶4 ratio by χ2 test as expected.All the above phenotypic and genotypic segregation data demonstrated the hypothesis that male fertility restoration of NJCMS2A was controlled by two pairs of duplicate dominant genes，which further verified the results of Bai and Gai.One F1：2population from the NJCMS2A×Zhongdou 5 cross was chosen randomly to tag the restorer gene of NJCMS2A with 903 pairs of SSR primers selected randomly from the soybean public genetic linkage map reported by Cregan et al.The result showed that one SSR marker，e.g.Satt135 was linked to the restorer gene of NJCMS2A.The recombination ratio of the restorer gene of NJCMS2A with Satt135 calculated from the maximum likelihood method was 0.1127，and converted into Kosambi’s genetic distance，was 11.47cM.In comparison with the integrated linkage map by Song et al.，the restorer gene of NJCMS2A was located on the linkage group D2.

References:

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Last Update: 2014-10-10