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Breakthrough of Two Questions on the Agrobacterium-mediated Soybean Cotyledonary Node Systems(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年01期
Page:
173-175，180
Research Field:
Publishing date:

Info

Title:
Breakthrough of Two Questions on the Agrobacterium-mediated Soybean Cotyledonary Node Systems
Author(s):
LI Wen-xia1; LI Wen-bin2; Lv Wen-he1; and NING Hai-long2
1College of Agronomy，Northeast Agricultural University，Harbin 150030，Heilongjiang;
2Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030，Heilongjiang，China
Keywords:
Soybean; Cotyledonary node; Multiple shoots elongation; Calli tissue
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.01.0173
Abstract:
The factors influencing soybean（Glycine max L.） cotyledonary node regeneration and detection from soybean cultivars “Heinong 35” were studied in this research so as to solve the two problems.L934orthogonal design was used to discuss effect of four key factors and levels on multiple shoot elongation.DNA for Southern blot were extracted from calli tissue which were induced from putative transgenic plants leaves.The results indicated that the better conditions for elongation were got when concentration of 6-Benzylaminopurine in subculture medium for shoot induction was dropped to 1/5 to the preceding shoot induction medium，and 50 mg L-1Glutamic acid，0.5 mg L-1Gibberellin A3 and 0.5 mg L-1Zeatin were added to shoot elongation medium；Southern blot signal indicated that calli tissue from leaves could extract enough genomic DNA.

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Last Update: 2014-10-10