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Comparing Different Methods for Isolating Fusarium from Soybean Rhizosphere Soil(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年01期
Page:
106-112
Research Field:
Publishing date:

Info

Title:
Comparing Different Methods for Isolating Fusarium from Soybean Rhizosphere Soil
Author(s):
LIU Jin-bo1; 2; XU Yan-li1; and WEI Wei1; 2; 3
1 Northeast Institute of Geography and Agricultural Ecology，CAS，Harbin 150081，Heilongjiang；
2 Graduate School，CAS，Beijing 100049；
3 Northesat Forestry University，The college of life science，Harbin 150040，Heilongjiang，China
Keywords:
Soybean; Rhizosphere soil; Fusarium; Isolation methods
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.01.0106
Abstract:
Fusarium root rot of soybean is one of the soil-borne diseases which is difficult to control.In soil，distribution of Fusarium is surprisingly diverse，which can efficiently influence the pathogenic Fusarium populations and host resistance.Isolation of Fusarium is very useful in the study of soil Fusarium diversity.Many methods for the isolation of Fusarium have been reported，but the efficiency was certainly different.In this paper，some related contents which affect the effeciency of Fusarium separation were researched，and dilution plate method and direct soil plating method were compared.The results showed that bacteria could be inhibited excellently when the concentration of antibiotic reached fourfold，but there was no significant impact on the number of fungi.Interestingly，it seems that different combination of antibiotics had effect on Fusarium isolation.Between colony forming units (CFU) of fungi and different sample volume，well linear relationship were detected in both sterile water and Water Agar treatment（R2>0.9).The isolation rate of Fusarium in sterile water treatment was 21.0%，a bit higher than that in Water Agar treatment，which was 10.0%.No significant effects on Fusarium isolation between two culture media were observed.Compared with Malachite Green Agar (MGA)，Peptone PCNB Agar (PPA) behaved higher isolation rate of Fusarium which was 20.93%.The difference between Fusarium isolation rates derived from two culture methods were significant.The isolation rate of Fusarium derived from direct soil plating method were all above 60.0%，which is significantly higher than that derived from dilution plate method which were all below 6%.

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Last Update: 2014-10-09