|Table of Contents|

Transformation of Stress Resistance Related Gene GmDREB into Soybean via Agrobacterium-mediation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年01期
Page:
47-51
Research Field:
Publishing date:

Info

Title:
Transformation of Stress Resistance Related Gene GmDREB into Soybean via Agrobacterium-mediation
Author(s):
WANG Ping1GAO Shi-qing2GUO Yong-lai3CHENG Xian-guo4WANG Gang5MA You-zhi2and JI Jing5
3Changchun Longyang Soybean Biologic Albumen Co.Ltd,Changchun 130062,Jilin;
4 Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;
5 Agriculture and biology engineering college,Tianjin University,Tianjin 300072,China
Keywords:
Soybean(Glycine maxL.)AgrobacteriumGmDREB geneImmature cotyledon
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.01.0047
Abstract:
Plant genetic transformation techniques have been important to improve crop varieties.Combined with traditional breeding process,molecular breeding techniques have showed bright prospects for improving agronomic characters in soybean.The somatic embryogenesis and basic resistance to PPT in immature cotyledon with 5 genotypes of soybean were tested.Plasmid prdGm-200 containing GmDREB gene was transformed into immature cotyledon of soybean via Agrobacterium-mediation.The main factors affecting Agrobacterium-mediated transformation were also investigated.The result showed that the somatic embryogenesis of immature cotyledon were different among 5 genotypes in soybean.The somatic embryogenesis in Dongnog 42 and Jilin 35 were higher than those in Heinong 41,Jiu 9568 and Jiu 9313.The immature cotyledon of Dongnog 42 and Jilin 35 were sensitive to PPT.The screening concentration of PPT was appropriate with 2-3 mg L-1.Transformation efficiency was also affected by concentration of Agrobacterium-EHA101 and inoculation time.OD 0.5-0.7 and 10-20 minutes of inoculation were helpful to transformation.The resistant somatic embryos and plantlets were identified after PPT screening,PCR and PCR~Southern detection.It was demonstrated that the GmDREB gene had been transformed into soybean.

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Last Update: 2014-10-09