|Table of Contents|

Comparison of the Genomic DNA Extraction Methods From Soybean Different Tissues(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年01期
Page:
42-46
Research Field:
Publishing date:

Info

Title:
Comparison of the Genomic DNA Extraction Methods From Soybean Different Tissues
Author(s):
WANG Zhen-dong; SUN Cang; and WANG Hui
Science and Technology Department of Shenyang Agricultural University，Shenyang 110161，Liaoning，China
Keywords:
Soybean genomic DNA; Extraction; SDS method; CTAB method
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.01.0042
Abstract:
It is important to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research.In order to study the soybean genome by PCR，RAPD，SSR etc.，improved SDS and CTAB method were used to extract the soybean genomic DNA from the soybean seeds and leaves.The DNA extraction effect of two different methods was compared by ultraviolet spectrophotometry，agarose electroforesis and the restriction enzyme digestion.The results showed that the extraction effect of the SDS method and the CTAB method to soybean genomic DNA was not obviously different for soybean leaves.However，the extraction effect of SDS method is much better than CTAB method for soybean seeds.The conclusion is that the SDS method is able to extract high quality and quantity soybean genomic DNA，which is suitable for various molecular operations.

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Last Update: 2014-10-09