|Table of Contents|

Optimization of Genetic Transformation System from Soybean Cotyledon Mediated by Agrobacterium(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年01期
Page:
26-32
Research Field:
Publishing date:

Info

Title:
Optimization of Genetic Transformation System from Soybean Cotyledon Mediated by Agrobacterium
Author(s):
JI Yue-mei1; 2; CHEN Shou-yi3; LI Ying-hui2; ZHANG Li-juan2; SONG Xiao-hua1; CHANG Ru-zhen2; and QIU Li-juan2
1Ningxia University Agricultural College，Yinchuan 750021，Ningxia;
2The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) / Key Laboratory of Germplasm & Biotechnology (MOA)，Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，100081 Beijing;
3The National Key Lab of Plant Genomics，Institute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，China
Keywords:
Soybean; Transformation; Cotyledonary-node; Na+/H+ antiporter; Regeneration
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2008.01.0026
Abstract:
Transgenic soybeans have been widely planted aboard，which leading the development of the transgenic crop in the world.Establishment of an efficient genetic transformation technology system will facilitate physiological and molecular biology studies as well as the improvement of cultivars in China. The objective of this study was to optimize the conditions of the cotyledon node system of Agrobacterium-mediated soybean transformation so that the transformation plants can be obtained.Four soybean genotypes of Zhongdou 32，Peking，Zaoshu 18 and Suinong 14 was used to introduce the wheat transcription factor Na+/H+antiporter into soybean genome by Agrobacterium-mediated transformation.The proportions of healthy explants，resistance tufty shoots and elongated shoots used as criteria for selection of proper conditions.The results indicated that the optimal system included in 0.5 mg L-1and 1.0 mg L-1of 6-BA for germination medium and shoot induction，2/3 cotyledon infected with 30 min for infection，3 days for co-culture，selection with 60 and 50 mg L-1Kan during the first and second shoot initiation stages. Based on the optimized condition positive transformed plants containing TaNHX2 was obtained，which tested by specific PCR，indicating that Na+/H+ antiporter gene was into the genome of soybean.The results indicated that genotypes mainly affected rates of transformation and regeneration.The rate of genetic transformation in cultivar Zhongdou 32 was 3.78%.

References:

[1]Cheng L H，Sun Y，and Liu S X.2001.The effects of genotype and phytohormone concentration on soybean plant regeneration.Chinese Bulletin of Botany，18(3):367-370(程林海，孙毅，刘少翔.2001.基因型和激素浓度对大豆植株再生的影响.植物学通报，18(3):367-370)

[2]Chen S Y.2004.High efficiency Agrobacterium-mediated transformation of soybean.Acata Botanica Sinica ，46(5):610-617
[3]Cheng T Y，Saka T，and VoqiI-Dinh T H.1980.Plant regeneration from soybean cotyledonary node segments in culture.Plant Science Letter，19:91-99
[4]Di R，Purcell V，Collins G B，and Ghabrial S A.1996.Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene.Plant Cell Reports，15:746-750
[5]Deng X Y，and Wei Z M.1998.Soybean conversion technology.Plant Physiology Communications，34:381-387 (邓向阳，卫志明.1998.大豆转化技术.植物生理学通讯，34:381-387) Li G L， [5]Qiao Y K， Yang S H，Jin Z X，and Li M G.2005.Study of the Agrobacterium-mediated transformation systems of soybean cotyledonary node.Acta Agronomica Sinica，31(2):170- 176(李桂兰，乔亚科，杨少辉，靳朝霞，李明刚.2005.农杆菌介导大豆子叶节遗传转化的研究.作物学报，31(2):170-176)
[6] Li M C，Cai Y，Zhao G L，Cai Y Q G L，Zhou H，Song W，and Xing L J.2006.Improvement of cotyledon node regeneration system in soybean(Glycine max)．Acta Agronomica Sinica，32(2):223-227(李明春，蔡易，赵桂兰，财音青格乐，周皓，孙伟，邢来君.2006.改良大豆子叶节再生体系的研究.作物学报，32(2):223 -227)
[7]Lin S Z，Cao Y P，Wei Z M，Ma X P，and Chen L Y.2005.Studies on shoots induced by 6-BA from cotyledonary-nodes and embryonic tips of soybean.Journal of Shanghai Jiaotong University，23(2):138-142(林树柱，曹越平，卫志明，马晓平，陈鲁勇.2005.6-BA 诱导大豆子叶节和茎尖出芽的研究.上海交通大学学报，23(2):138-142)
[8]Olhoft P M，Flagel L E，Donovan C M，and Somers D A.2003.Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method.Planta，(216):723-735
[9]Olhoft M，and Somers D A.2001.L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells．Plant Cell Reports，20:706-711
[10]Par rott W A，Williams E G，and Hildebrand D P.1989.Effect of genotype on somatic embryogenesis form immature cotyledons of soybean.Plant Cell Tissue and Organ Culture，16(1):15-21
[11]Paz M M，Martinez J C，Kalvig A B，Fonger T M，and Wang K.2006.Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation.Plant Cell Reports，25:206-213
[12]SatoS，Newell C，and Kolacz K.1993.Stable transformation via particle bombardment in two different soybean regeneration systems.Plant Cell Reports，12:408-413
[13]Wright M S，Koehler S M，and Hichee M A.1986.Plant regeneration by organogenesis in -Glycine max-.Plant Cell Reports，5:150-154
[14]Wright M S，Williams M H，and Pierson R E.1987.Initiation and propagation of Glycine max L.Merr.Plants from tissue -cultured epicotyls.Plant Cell Tissue and Organ Culture，(8):83-90
[15]Wang P，Wang G，Ji J，Zhou Z M，Guo W，La B，and Wu Y.2004.Genetic Transformation of Immature cotyledon via Agrobacterium-tumefaciens in soybean.Soybean Science，23(2):86-90(王萍，王罡，季静，周智明，郭威，拉巴，吴颖.2004.农杆菌介导大豆未成熟子叶的遗传转化.大豆科学，23(2):86-90)
[16]Wang S J，Wu Y H，Wang H Y，and Liu Z Z.1999.Studies on the regeneration of tixxue culture of different exophytes in soybean.Journal of Shenyang Agricultural University，30(3):255-259(王升吉，吴元华，王洪岩，刘忠智.1999.大豆不同外植体组织培养及再生研究.沈阳农业大学学报，30(3):255-259)
[17]Wang X C，Wang G，Wang P，Ji J，and Liu S Q.2006.Genetic transformation of somatic embryos via Agrobacterium tumefaciens in soybean.Chinese Agricultural Science Bulletin，22(4):40-43(王晓春，王罡，王萍，季静，刘尚前.2006.农杆菌介导法转化大豆体细胞胚获得转基因植株.中国农学通报，22(4):40-43)
[18]Xue R G.2006.Agrobacterium-mediated transformation of soybean germinating cotyledonary node cells.Acta Agronomica Sinica，32(8):1188-1192(薛仁镐.2006.用根癌农杆菌介导法转化大豆萌动子叶节细胞.作物学报，32(8):1188-1192)
[19]Xiao W Y，and Wang L Z.1994.David cotyledons of soybean pods protoplast culture and plant regeneration.Acta Agronomica Sinica，20(6):665-669(肖文言，王连铮.1994.大豆幼荚子叶原生质体培养及植株再生.作物学报，20(6):665-669)
[20]Yang R Z，Tan Y M，Li S，Zhang M Q，and Chen R K.2003.Studies on regeneration of soybean cotyledon node and Agrobacterium-mediated genetic transformation.Southwest China Journal of Agricultural Sciences，16(4):33-38(杨荣仲，谭裕模，李松，张木清，陈如凯.2003.大豆子叶节再生及农杆菌介导转化研究.西南农业学报，16(4):33-38)
[21]Yuan Y，Liu D P，Zheng P H，Xu W J，and Li H L.2001.Study on plant regeneration from soybean culture.Soybean Science，20(2):9–13(袁鹰，刘德璞，郑培和，徐文静，李海龙.2001.大豆组织培养再生植株研究.大豆科学，20(2):9-13)
[22]Zhang Z Y，Xing A Q，Staswick P，and Clemente T E.1999.The use of glufosinate as a selective agent in Agrobacterium-mediated transformation of soybean.Plant Cell.Tissue and Organ Culture，56:37-46

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Last Update: 2014-10-08