|Table of Contents|

DETERMINATION OF MONODESMOSIDE SOYASAPONINS FROM SOYBEAN HYPOCOTYL BY HPLC(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年06期
Page:
943-945
Research Field:
Publishing date:

Info

Title:
DETERMINATION OF MONODESMOSIDE SOYASAPONINS FROM SOYBEAN HYPOCOTYL BY HPLC
Author(s):
YIN Xue-zhe1; SHEN Ming-hua1; QUAN Ji-shu1; KUDOU Shigemitsu2
1.Medical College of Yanbian University，Yanji 133000；
2.Institute of Food Factors Science for Health，Aomori 030-0842，Japan
Keywords:
Soybean Hypocotyl; Mono-desmoside Soyasaponins
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.027
Abstract:
Soyasaponins have many pharmacological effects.The determination of soyasaponins contents is the basis of the pharmacological experiments.In the current experiment mono-desmoside soyasaponins in soybean sample were detected by a high performance liquid chromatographic-differential refractive index detective (HPLC-dRI) method.The chromatographic condition was as fellows：ODS-AM-303 column (YMC，4.6 mm 250 mm，5 μm) with the temperature of 40℃，acetonitrile-water (40∶60) containing 0.1% trifluoroacetic acid as mobile phase with the flow rate of 1 mL min-1.The method was proved to be linear in ranges of 2.24-11.2，2.35-11.8，1.58-7.92，2.01-10.1，1.38-6.88 and 1.62-8.12 μg for Ba，Bb，Bd，Be，αg and βg，respectively；the recoveries were 92.5%，93.8%，94.1%，95.8%，93.4% and 94.2% with RSD of 3.22%，3.18%，4.01%，3.53%，4.07% and 4.28% for Ba，Bb，Bd，Be，αg and βg，respectively.The method is accurate and reproducible，and suitable for the determination of mono-desmoside soyasaponins.

References:

［1］杨光，顾雪峰，韩俊.大豆皂甙的提取、生理功能及应用进展[J].大豆通报，2005，6:23-26.

［2］Rodrigues H G，Diniz Y S，Faine L A，et al.Antioxidant effect of saponin：potential action of a soybean flavonoid on glucose tolerance and risk factors for atherosclerosis[J].International Journal of Food Sciences and Nutrition，2005，56:79-85.
［3］Ishii Y，Tanizawa H.Effects of Soyasaponins on lipid peroxidation through the secretion of thyroid hormone[J].Biological & Pharmaceutical Bulletin，2006，29:1759-1763.
［4］Yoshikoshi M，Yoshiki Y，Okubo K，et al.Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts [J].Planta Medica，1996，62(3):252-255.
［5］Kinjo J，Imagire M，Udayama M，et al.Structure-hepatoprotective relationships study of soyasaponins I-IV having soyasapogenol B as aglycone [J].Planta Medica，1998，64(3):233-236.
［6］袁晓洁, 郭英, 孙维琦,等.大豆异黄酮与大豆皂甙抗疲劳作用[J].中国公共卫生，2007，23(3):327-328.
［7］茹湘波，谷克仁，耿敏，等.大豆皂甙分析检测方法研究进展[J].粮食与油脂，2006，12:40-42.
［8］Kudou S，Tonomura M，Tsukamoto C，et al.Isolation and structural elucidation of the major genuine soybean saponin[J].Bioscience，Biotechnology，and Biochemistry，1992，56(1):142-143.
［9］田晶，翟滨.脱脂大豆中B组大豆皂甙V的HPLC-MS检测[J].食品科学，2002，23(12):101-103.
［10］魏静，张春红，刘长江.比色法测定大豆总皂甙的研究[J].粮油加工，2006，10:60-62.
［11］薛锦峰，闫子鹏，郭丰盛.紫外分光光度法测定大豆中的总皂甙[J].中国油脂，2006，31(10):77-78.

Memo

Memo:
-
Last Update: 2014-10-20