|Table of Contents|

PURIFICATION AND SEPARATION OF SOYBEAN ESTERASE FOR PESTICIDE RESIDUE DETECTION(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年06期
Page:
935-938，942
Research Field:
Publishing date:

Info

Title:
PURIFICATION AND SEPARATION OF SOYBEAN ESTERASE FOR PESTICIDE RESIDUE DETECTION
Author(s):
ZHOU Yan-li; LI Jian-ke; WEN Yan-xia
College of Food Engineering and Nutritional Science，Shaanxi Normal University,Xi’an 710062
Keywords:
Pesticide residues; Rapid detection; Soybean Esterase; Purification; Separation
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.025
Abstract:
To search a new esterase for pesticide residue detection from soybean seeds，the soybean seed powder was dealt with crude extraction，differential centrifugation，ammonium sulfate salting-out to purify soybean esterase.The purified enzyme was then separated by DEAE-32 ion exchange column chromatography and Sephadex G-100 gel filtration column chromatography.Then，the inhibition to Organophosphate and Carbamate pesticides of the esterase was observed.Three soybean esterase of electrophoresis purity were obtained，marked with E1，E2and E3.Rusults indicated that E1had no sensitivity (inhibition) to Organophosphate and Carbamate pesticide while Ewas better sensitive than E3.Hence，a new esterase for pesticide residue detection from soybean seeds and a better purification method were obtained.

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Last Update: 2014-10-19