|Table of Contents|

CLONING AND SEQUENCE ANALYSES OF 11S GLYCININ Gy7 GENE FROM SOYBEAN NANNONG 87C-38(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年06期
Page:
840-846,852
Research Field:
Publishing date:

Info

Title:
CLONING AND SEQUENCE ANALYSES OF 11S GLYCININ Gy7 GENE FROM SOYBEAN NANNONG 87C-38
Author(s):
GU Ting-yu; LI Jian-yue; MI Dong; XIAO Gang
College of Life and Environment Sciences，Shanghai Normal University，Shanghai 200234
Keywords:
Soybean; 11S glycinin Gy7 gene; 11S glycinin Gy7 cDNA; Clone; Sequence analyses
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.007
Abstract:
Rice and wheat are main crops for human consumption，but the seeds in those crops generally lack lysine. Soybean has an abundant storage of protein and lysine，which could help to make up for the lack of lysine in rice and wheat. 11S Glycinin Gy7 gene and its cDNA of soybean Nannong 87C-38 were cloned，independently，with the help of the molecular biotechnology. After being compared the two sequences with Genbank (AF319776 and AF319777)by vector NTI software，their identities were 99% and 99.6%. Interestingly，most differences of the nucleotide acid between Gy7 cDNA and Genbank AF319777 were located in the third extron. Thus we presume that the amino acid coded by the first，second and fourth extrons are more stable and they play a more important role in forming specific structures of the G7 polypeptide than the third extron. According to the genetic code，we presumed the amino acid coded by Gy7cDNA. There were two amino acid differences from Genbank AF319777.This superior gene cloned in this research could lay the foundation for improving nutritional quality of the crops such as rice and wheat.

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Last Update: 2014-10-19