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Developing DNA Markers for Assisting Selection of Field Weathering Resistance in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年06期
Page:
820-827
Research Field:
Publishing date:

Info

Title:
Developing DNA Markers for Assisting Selection of Field Weathering Resistance in Soybean
Author(s):
YE Chang-rong1; Prapa Sripichitt2; Vipa Hongtrakul3; Sunanta Juntakool2; Arom Sripichitt4and Shu Fukai1
1.School of Land，Crop and Food Sciences，University of Queensland，Brisbane 4072，Australia；
2.Department of Agronomy，Kasetsart University，Bangkok 10900，Thailand；
3.Department of Genetics，Kasetsart University，Bangkok 10900，Thailand；
4.Department of Plant Production Technology，King Mongkut’s Institute of Technology Ladkrabang，Bangkok 10520，Thailand
Keywords:
Soybean; Field weathering resistance; Bulked segregant analysis; Quantitative trait locus; Marker assisted selection
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.06.004
Abstract:
The deterioration of seed vigor as well as viability，due to high temperature and high relative humidity during the stages of the post-maturation and pre-harvest period is referred to as field weathering. Field weathering is the main limitation for producing high quality soybean seeds in the tropics and subtropics. The purpose of this study is to identify DNA markers linked to the field weathering resistant genes，and to develop markers for assisting selection in breeding program. The field weathering resistance of soybean variety Chiangmai 60 (susceptible)，GC10981 (resistant)and 139 F2progenies derived from the cross of CM60/GC10981 was tested by modified incubator weathering and the controlled deterioration treatment. The seeds germination and viability of F2progenies showed normal distribution under both treatments. It hinted that the field weathering resistance was controlled by polygene. According to the seeds germination and viability of the F2progenies，six extremely resistant plants and seven extremely susceptible plants were pooled for bulk segregant analysis by AFLP markers. Five field weathering resistance linked polymorphism were identified from 2 162 AFLP markers. The 5 DNA fragments were cloned and sequenced. PCR primers were designed from the sequences to amplify the related DNA fragment from the genomic DNA of F2progenies. It was found that marker Eaag/Mcac-233 and Eact/Mctt-157 were in the same linkage group with a genetic distance of 25.8 cM. A major QTL controlling the field weathering resistance was identified between these two markers. The QTL located at 14 cM from marker Eaag/Mcac-233 and explained 29.7% of the variation in field weathering resistance. These two DNA markers have been used for assisting selection in breeding program as an attempt. Seven F2progenies were selected and backcrossed to CM60 using the developed markers in combination with field weathering resistance characters. The germination and viability of 18 BC1F1progenies (41.9%）were higher than the mean of CM60 and GC10981 by controlled deterioration test. It is potentially possible to use these markers for assisting selection in breeding programs that focus on seed quality in the tropics.

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Last Update: 2014-10-19