In 2006,the resistance of new soybean cultivars(lines) of Heilongjiang Province to Frogeye leafspot was identified by inoculation with Colletotrichum lagenarium,two highly-resistant germplasms were selected,they were Dongnong 03-8784,Sui 00-1053,accounted for 2.78% of total identified soybean lines.Eight resistant lines were also selected,they were Hajiao 20-5489,Heihe 00-1386,Ken 01-3273,Nongda 05089,Nongda 25146,Nongda 25099,Nongda 25710,Sui 99-3213,accounted for 11.11% of total identified soybean lines,Forty-five middle-resistant lines were also selected,accounted for 62.50% of total identified soybean lines,The others were susceptible or highly-susceptible ones.