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A SIMPLE DETACHED LEAF CULTURE TECHNIQUE FOR STUDY OF SOYBEAN FOLIAR DISEASE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年05期
Page:
740-743
Research Field:
Publishing date:

Info

Title:
A SIMPLE DETACHED LEAF CULTURE TECHNIQUE FOR STUDY OF SOYBEAN FOLIAR DISEASE
Author(s):
MO Jian-you 1; GUO Tang-xun 1; 3; Navi S S2; LI Xun2; YANG Xiao-bing2
1Plant Protection Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007；
2Department of plant pathology，Iowa State University，Ames，Iowa 50010，USA；
3Agricultural College，Guangxi University，Nanning 530004
Keywords:
Soybean; Detach leaf; Root regeneration; Soybean rust(Phakopsora pachyrhizi Syd); Soybean powdery mildew (Microsphaera diffusa); Susceptibility
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.05.021
Abstract:
Detached leaves were used to find a simple and reliable method for studying the foliar diseases of soybean.The result showed the root generated from soybean detached leaf as long as 16.8~41.2 cm(with mean length of more than 24 cm)after culturing for 30 days，and remained green for additional 30 days in a pot with soil.It demonstrated that detached leaves produced identical or similar responses to leaves from the whole plants when inoculated with two fungal pathogens soybean rust(Phakopsora pachyrhizi Syd) and soybean powdery mildew(Microsphaera diffusa).This method could be applied for other foliar diseases study of soybean with easier controlled experiment condition and without more time and space.

References:

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Last Update: 2014-10-20