PLANT REGENERATION FROM COTYLDEONARY NODES AND EMBRYONIC TIPS OF SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Title:: PLANT REGENERATION FROM COTYLDEONARY NODES AND EMBRYONIC TIPS OF SOYBEAN

Author(s):: LI Hai-yan; WU Xiao-xia; LIU Miao; HAN Ying-peng; Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030

Abstract:: In order to obtain high efficiency soybean regeneration system，cotyledonary nodes and stem tips of soybean Hefeng 35 and Peking was used as explants.The disinfect ways of mercuric chloride and chlorine gas were compared，and the effect of 6-BA and 2,4-D on the regeneration of soybean cotyledonary node and stem tip were investigated.The results showed chlorine gas was a better disinfection method.The best concentration of 6-BA and 2,4-D was 1.0 and 2.0 mg L^-1in germination medium respectively.The optimum concentration of 6-BA and 2,4-D was 3.0 and 3.5 mg L^-1in regeneration of stem tips.And the best inducing time was 24-28 h.

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Last Update: 2014-10-20