|Table of Contents|

TRANSFORMING lkt50 GENE TO SOYBEAN BY POLLEN TUBE PATHWAY(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年05期
Page:
653-656
Research Field:
Publishing date:

Info

Title:
TRANSFORMING lkt50 GENE TO SOYBEAN BY POLLEN TUBE PATHWAY
Author(s):
LI Wen-xia1; LI Wen-bin2; NING Hai-long2; Lv Wen-he1
1College of Agronomy，Northeast Agricultural University，Harbin 150030;
2Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030
Keywords:
Soybean; Pollen-tube pathway; lkt50 gene
PACS:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.05.003
Abstract:
lkt50 gene was introduced into soybean cultivar Dongnong 48 and strain Dongnong 96-02 via pollen tube pathway.The transformed plants were primary screened from seedling by spraying kanamycin，and further the transformed plants was determined by molecular biological methods including PCR，Southern blot and RT-PCR.The results indicated that the identification of 14 Km resistant plants was determined by PCR with the use of the primers of nptII and lkt50，and seven and two plants were found to be positive respectively.Furthermore，the detections by Southern blot and RT-PCR revealed that the two plants were found to be positive.The pollen tube pathway is an effective，feasible method for soybean transgene.

References:

［1］Griffin D.Economic impact associated with respiratory disease in beef cattle[M].In:Vestweber，J.，St.Jean，G.(Eds.)，Veterinary Clinics of North America:Food Animal Practice.Saunders，Philadelphia，1997，367-377.

[2]Cho H J，Jericho K W F.Induction of immunity against pneumonic pasteurellosis following experimental infection in calves[J].Candian Journal of Veterinary Research，1986，50：27-31.
[3]Sutherland A D，Donachie W.Cytotoxic effect of serotypes of Pasteurella haemolytica on sheep bronchoalveolar macrophages[J].Veterinary Microbiology,1986，11：331-336.
[4]Lee R W H，Strommer J，Hodgins D，et al.Towards development of an edible vaccine against bovine pneumonic pasteurellosis using transgenic white clover expressing a Mannheimia haemolytica Al Leukotoxin 50 fusion protein[J].Infection and Immunity，2001，69(9)：5786-5793.
[5]刘德璞，袁鹰，唐克轩，等.大豆花粉管通道技术转化雪花莲凝集素(GNA)基因[J].分子植物育种，2006，5(4):663-669.
[6]刘德璞，袁鹰，庄炳昌.外源DNA导入大豆变异后代的SOD同工酶分析[J].大豆科学，1991，10（3）：194-199.
[7]刘德璞，廖林，袁鹰，等.导入外源DNA获得抗SMV大豆[J].大豆科学，1997，16(4)：277-282.
[8]刘德璞，袁鹰，王成武，等.导入外源DNA大豆后代的抗虫性鉴定与筛选[J].大豆科学，2002，21(4)：245-249.

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Last Update: 2014-10-20