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EXPRESSION OF SOYBEAN MP2 PROTEIN(LEA3)CONFERS SALT TOLERANCE IN SACCHAROMYCES CEREVISIAE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年04期
Page:
467-472
Research Field:
Publishing date:

Info

Title:
EXPRESSION OF SOYBEAN MP2 PROTEIN(LEA3)CONFERS SALT TOLERANCE IN SACCHAROMYCES CEREVISIAE
Author(s):
LI Ran-hui; LIU Yun; ZHENG Yi-zhi
College of Life Sciences，Shenzhen University，Shenzhen 518060
Keywords:
Soybean; LEA3 protein; PM2protein; Salt tolerance; Yeast
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.04.004
Abstract:
Soybean PM2 protein belongs to the family of group 3 LEA（late embryogenesis abundant）proteins.Nucleotide acid fragment of PM2 gene was amplified by PCR reaction using the plasmid pET28a/PM2 as the template.The yeast expression plasmid of pYES2/PM2 was constructed and then transformed into yeast to create recombinant INV/PM2.The results of SDS-PAGE analysis showed that a specific protein band of 50 kDa was expressed in the recombinant yeast of INV/PM2.The protein was close to the theoretic molecular weight of PM2 protein and was identified as PM2 protein by LC-ESI-MS assay.The growth curves of recombinant INV/PM2 and INV/pYES2 with an empty vector were generated under the non-stress，high salinity (1.8mol/L NaCl) or osmotic (2mol/L sorbitol) conditions，respectively.The growth rates of the recombinants with PM2 protein under the non-stress condition indicated that the expression of PM2 protein is not deleterious to the growth of yeast.The yeast cells expressing PM2 protein showed shorter lag period when transferred to a medium containing high salinity as compared to the control.This suggests that the expression of PM2 protein could confer salt tolerance in yeast.But no obvious growth improvement was observed in a high sorbitol medium between the recombinant INV/PM2 and INV/pYES2.

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Last Update: 2014-10-20