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CLONING AND SEQUENCE ANALYSIS OF PROMOTER OF SEED Em GENE(LEA1) IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年04期
Page:
454-459
Research Field:
Publishing date:

Info

Title:
CLONING AND SEQUENCE ANALYSIS OF PROMOTER OF SEED Em GENE(LEA1) IN SOYBEAN
Author(s):
LIU Guo-bao; ZHENG Yi-zhi
College of Life Sciences，Shenzhen University，Shenzhen 518060
Keywords:
Glycine max; Em gene; LEA gene; Induced promoter; Chromosome walking
PACS:
S565.1.Q785
DOI:
10.3969/j.issn.1000-9841.2007.04.002
Abstract:
According to the sequence of soybean seed Em gene from Genebank，two reverse primer was designed and synthesized.A DNA fragment of 836bp upstream of the coding sequence of Em gene was amplified by chromosome walking with the genomic DNA of Glycine max as the template.Sequence analysis showed that the fragment was a novel DNA sequence.Using Bio.Informatics software,it was found the fragment contained the core elements of promoter including TATA-box and CAAT-box,so that it could be possibly the promoter sequence of Em gene.In addition,the fragment contained some putative cis-elements,such as a DRE1-motif,two ABRE-motifs,an AG-motif and an ELRE-motif etc,constituting the drought/salt-induced promoter activity.Then,it is found that the fragment contained two RY-repeat motifs and a TGTCACA-Motif,indicating the potential seed-specific promoter activity.Based on above preliminary analysis,it is suggested that the fragment might be the promoter of Em gene with induced promoter activity and seed-specific promoter activity.

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Last Update: 2014-10-20