|Table of Contents|

SOYBEAN [GLYCINE MAX (L) MERR]TRANSFORMATION OF HERBICIDE RESISTANT GENE(BAR) VIA POLLEN TUBE PATHWAY(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年03期
Page:
310-314
Research Field:
Publishing date:

Info

Title:
SOYBEAN [GLYCINE MAX (L) MERR]TRANSFORMATION OF HERBICIDE RESISTANT GENE(BAR) VIA POLLEN TUBE PATHWAY
Author(s):
LIU Zhao-jun1;  LI Tie1;  LIU Li-yan1; LIU Qi1;  WANG Xun1; MA Qi-hui2
1.Biotechnology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin, 150086;
2.Heilongjiang Academy of Agricultural Sciences,Harbin,150086
Keywords:
Soybean[Glycine max(L) Merr]; Pollen tube pathway; bar gene
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.03.004
Abstract:
Plasmid pPTN140 containing bar gene was transferred into eight soybean cultivars via pollen tube pathway, 1384 flowers were treated and 1164 seeds were harvested-Eight plants of three cultivars were survived after two times Basta screeningPositive results were given by PCR and PCR- Southern certification on the eight herbicide resistant plants-Southern blotting shown that the eight plants integrated different copies bar gene, two of them integrated single copy gene and other six plants integrated three to eight copies gene respectively-Genetic analysis was done to the offspring of the eight plants and indicated that there were still herbicide resistant plants in D1-D3generation, which suggested that bar gene could inherit to the transformed offspring and functioned in it.Genetic analysis of herbicide resistance indicated that there was no regularity within lines or among lines, and the segregation of herbicide resistance didn’t obey the Mendelian Law.It also indicated that there were co-inhibiting phenomena because of multiple copies gene integrated in pollen tube pathway.By now, 82 stable herbicide resistant lines of D3 generation had been obtained.The research showed that it was feasible to transform soybean via pollen tube pathway

References:

［1］周光宇, 龚蓁蓁, 王自芳.远缘杂交的分子基础[J]遗传学报1979，6(4)：405-413.

[2]王才林，赵凌，宗寿余，等.用花粉管通道法将 bar基因导入水稻获得可遗传的转基因植株[J.江苏农业学报，2002，18(3)：129-133.
[3]黄骏麒,钱思颖，刘桂玲，等外源海岛棉DNA导致陆地棉性状的变异[J].遗传学报，1981，8(1)：56-62
[4]雷勃钧，君光初，卢翠华，等.外源DNA直接导入大豆的研究[J].大豆科学，1991，10(1)：58-63.
[5]崔岩，杨庆凯，周思军.利用花粉管通道技术导入大豆抗病虫目的基因[J].生物技术，2002, (6)：5-7.
[6]丁群星，谢友菊，戴景瑞，等.用子房注射法将 Bt毒蛋白基因导入玉米的研究[J].中国科学（B辑），1993，23(7)：707-713.
[7]裴新梧，崔凯荣，孔英珍，等.导入高梁DNA选育丰产、抗逆小麦新品系及其RAPD分子验证[J].兰州大学学报(自然科学版)，1999，35(2)：130-135.
[8]刘德璞，李长有.导入外源DNA获得抗SMV大豆品系[J].松辽学刊(自然科学版)，1995，3：40-43.
[9]杜海军，芦兴武，赵恩鹏，等.花生DNA导入大豆的后代POD、SOD活性及其同工酶谱分析[J].东北师大学报(自然科学版)，1996，1：87-89.
[10]徐香玲，邹联沛，刘伟华，等.向大豆导入几丁质酶基因的初步研究[J].大豆科学，1999, (2)：101-108.
[11]周思军.大豆抗虫基因转移及转化系统优化研究[D].东北农业大学博士学位论文，2000.
[12]章善庆，童汉华，薛锐，等.利用bar基因导入恢复系提高杂交稻纯度的尝试[J].中国农业科学，1998，31(6)：33-37.
[13]王关林，方宏筠.植物基因工程原理与技术[M].北京：科学出版社，1998：600-601.
[14]萨姆布鲁克J，拉塞尔 D.W著.黄培堂等译.分子克隆实验指南, 第三版[M].北京：2002，科学出版社，502-512.
[15]Peng J, Wen F, Lister R M, et al-Inheritance of gus and neo genes in transgenic rice[J].Plant Molecular Biology, 1995, 27：91-104.
[16]Wang Z H, Cui H R, Shu Q Y, et al.Genetic analysis of transgenes in the progenies of Bt rice cross to conventional rice varieties[J].Heredities, 2000, 22(5)：309-312.
[17]刘昭军.大豆外缘DNA直接导入技术及育种应用[J].大豆科学，2004， 4：301-306.
[18]刘昭军，李希臣，刘丽燕，等.大豆白化致死突变系的发现与分析[J].大豆科学，2005，3：229-231.
[19]曾君祉，吴有强，王东江，等.花粉管通道(或运载)法转化的植株后代遗传表现及转化机理的探讨[J].科学通报，1998，43(6)：561-566

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Last Update: 2014-10-22