|Table of Contents|

DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
273-275
Research Field:
Publishing date:

Info

Title:
DETERMINATION OF SOYBEAN ISOFLAVONES WITH ULTRA-VIOLET SPECTROPHOTOMETRY
Author(s):
HUANG Yun1; CU I Li-jian2; DOU Yu-hong2; WANG Xin-guo2; NIU Li-ying2
1.Department of Pharmacognosy,Hebei Medical University,Shijiazhuang 050017；
2.Department of Traditional Chinese Medicine,Hebei Medical University,Shijiazhuang 050091
Keywords:
Soybean isoflavones; NaOH; UV spectrophtometry; Quantitative determination
PACS:
TS207.3
DOI:
10.3969/j.issn.1000-9841.2007.02.032
Abstract:
A UV spectrophotometry used for determination of soybean isoflavones was established.Genistein was selected as the reference substance.Based on the reaction of genistein and NaOH,which has the maximum absorption in 271 nm wavelength,ultra-violet spectrophotometry method was used for determination of isoflavones in Glycine max(L.) Merr..The total content of isoflavones in soybean extracts was 4.784 mg/g with mean recovery 100.9%,and the RSD was 2.0%.The method is suitable for the routine analysis and quality control of the soybean and function food with easy-to-handle,good accuracy and reproducibility.

References:

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Last Update: 2014-10-22