PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2007年02期

Page:

250-253

Research Field:

Publishing date:

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Title:

PREPARATION AND QUALITY EVALUATION OF SOYBEAN PHOSPHATIDYLCHOLINE AS LIPOSOMAL MATERIAL

Author(s):

MU Xiao-mei¹; ZHONG Zhen-sheng²; YANG Mei¹

1.Department of Chemistry & Chemical Engineering,Zhongkai University of Agriculture and Technology,Guangzhou 510225；

2.College of Chemistry,South China University of Technology,Guangzhou,510641

Keywords:

Soybean phosphatidylcholines; Solvent extraction; Supercritical carbon dioxide extraction; and liposome

PACS:

TS224.4

DOI:

10.3969/j.issn.1000-9841.2007.02.027

Abstract:

High-purity soybean phosphatidylcholines(SPC) as liposomal material was prepared from soybean phospholipids with a method combined super critical carbon dioxide extraction and solvent extraction. The results of the quality evaluation showed that the SPC was separated with94.98% purity,the content of aceton-insoluble matter,hexane-insoluble matter,heavy metals and arsenic were respectively 99%,0.18%,2.0 10^-5% and 2.1 10^-6%,the acid value was 20.6 mg of potassium hydroxide,loss on drying of SPC was 0.63%.The liposome made with SPC showed spherical shape,its main particles was164 nm ,Zeta potential was -28.50 mv,and the entrapment efficiency was 32.43%.

References:

［1］Bangham A D,Horne R W. Negative staining of phospholipids and their structure modification by surface active agents as observed in electron microscope[J].Journal of Molecular Biology,1964,(8): 660-668.

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