|Table of Contents|

DETERMINATION OF ISOFLAVONES CONTENT IN SOYBEAN SEEDS BY CAPILLARY ELECTROPHORESIS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
230-234,244
Research Field:
Publishing date:

Info

Title:
DETERMINATION OF ISOFLAVONES CONTENT IN SOYBEAN SEEDS BY CAPILLARY ELECTROPHORESIS
Author(s):
LI Bo-tao; HAN Hang-ru; MA Hao; ZHANG Zhan-qin; ZHANG Xin; WANG Li-qun
State key Laboratory of Crop Genetics and Germplasm Enhancement National Center for Soybean Improvement,Ministry of Agriculture Nanjing Agricultural University,Nanjing,210095
Keywords:
Capillary electrophoresis;  Soybean isoflavones;  Content determination;  Optimization of electrophoretic conditions
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.02.023
Abstract:
Isoflavones is an important biofunctional substance. Capillary electrophoresis with a uncoated fused-silica capillary tube (50 cm 75 μm.i.d,effective length 42 cm) was used to optimize soybean isoflavones analysis. The optimum electrophoresis conditions were as follows: 40 mmol/L sodium borate containing 5% methanol as the running buffer(pH=10.0),electrophoretic voltage being 16.5 kV,temperature being 25 OC and detecting wavelength being 254 nm. Under the optimum conditions,6 kinds of isoflavones could be clearly separated within 21 minutes. The corrected peak areas of isoflavones increased linearly with components of isoflavones. RSD of the peak area and holding time of 6 isoflavones were 0.5%~2.4% and 1.8%~3.8%,respectively,and the recoveries were ranged from 97.2% to 103.3%. The biochanin was used as an internal standard and could increase the recurrence of experiment. The newly-built method is sensitive,simple,economical and well recurrent,and can be used for the determination of isoflavones components in soybean seed.

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Last Update: 2014-10-21