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A SENSITIVE MOLECULAR MARKER SSR ASSOCIATED WITH RESISTANT GENE TO HETERODERA GLYCINES(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
204-207,212
Research Field:
Publishing date:

Info

Title:
A SENSITIVE MOLECULAR MARKER SSR ASSOCIATED WITH RESISTANT GENE TO HETERODERA GLYCINES
Author(s):
WANG Hui1; YU Bai-shuang2; DUAN Yu-xi3; CHEN Li-jie3
1.Department of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110161;
2.Soybean Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086;
3.Department of Plant Protection, Shenyang Agricultural University, Shenyang 110161
Keywords:
Soybean; Heterodera glycines; BSA; Resistant Gene;  SSR Molecular marker
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.02.018
Abstract:
Soybean cyst nematode is one of the most serious diseases in the world soybean areas. The cross combination was made by the resistant soybean cultivar Xiaoliheidou as male parent and the susceptible Liaodou 10 as female parent. In the F2population the resistance was identified to Heterodera glycines Race 3 for every plant and separated the DNA from each plant. F2populations of Liaodou 10×Xiaoliheidou were analyzed with 204 pairs of SSR. There were 15 pairs of SSR primer with polymorphism. A 172 bp or a 176 bp SSR band was obtained which correlated with resistant gene to Heterodera glycines race 3 by primer Satt187 for the populations. They were codominance markers. The ratio of separation between F2population was 1:2:1. Satt187 was applied to identify and select F2population of Liaodou 10 and Xiaoliheidou. Satt187-176 bp appeared in resistant plants, and Satt187-176 bp and Satt187-172 bp could be both tested in susceptible plants, which indicated this marker was good for breeding in molecular markers.

References:

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Last Update: 2014-10-21