RESEARCH ON THE OPTIMUM CONDITIONS FOR SOYBEAN CALLUS SUBCULTURE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Author(s):: ZHANG Yu-song; KOU Wei-cai; Xian Engineering College of Armed Police Force,Xi-an,710086

Abstract:: In this paper, the subculture of soybean were studied from the aspects of basic media, culturing conditions, phytohormones etc.The optimum conditions for soybean callus subculture were as follows: Growth status of callus was better on MS medium supplemented with BA0.5 mg/L, 2,4-D2.0 mg/L, the callus yield and increasing times were the highest, it can reached 11.23 g/Flask and 10.23. The suitable pH value of medium was among 5.8~6.0. The callus cultured in light(1000~1500Lx，12 h/d) for 21d were suitable for the callus growth.

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Last Update: 2014-10-21