|Table of Contents|

ISOLATION OF BACTERIA IN WILD SOYBEAN RHIZOSPHERE AND STUDY ON ITS FUNCTION TO REDUCE DISADVANTAGE OF SOYBEAN CONTINUOUS CROPPING(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
176-180
Research Field:
Publishing date:

Info

Title:
ISOLATION OF BACTERIA IN WILD SOYBEAN RHIZOSPHERE AND STUDY ON ITS FUNCTION TO REDUCE DISADVANTAGE OF SOYBEAN CONTINUOUS CROPPING
Author(s):
LIU Zhao-jun; WANG De-guo;  LI Tie;  LIU Li-yan;  LEI Bo-jun;  LI Zhu-gang
1.Biotechnology Research Center of Heilongjiang Academy of Agricultural Sciences;
2.Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang ,Harbin 150086
Keywords:
Glycine soja; Microbe; Soybean continuous cropping
PACS:
S565.1;S154.3
DOI:
10.3969/j.issn.1000-9841.2007.02.012
Abstract:
Five bacterial strains were isolated from wild soybean rhizosphere soil in medium contained only one kind of carbonaceous organic compound. They could digest phthalate, malonic acid, acetic acid, propionic acid and benzene and were named HK14-2, HK26-2, HN31-1, HK46-2 and HK53-1, respectively. Through single colony culture, microscope observe, Gram’s dying and PCR-DGGE electrophoresis, it was showed that all of the screened five microbe were bacteria, HK26-2 was bacillus and others were coccus, and the isolated bacteria strain was pure. Test of colony standoff in dish proved the five bacteria could resist soybean pathogenic fungi(Rhizoctonia.solani). Potted soybean treated with different bacteria showed that the five bacteria could decrease the occurrence of soybean root rot, and the highest control effectiveness was 69.5% with compound bacteria of HN31-1 and HK53-1. Compared with the control, statistical data of taproot length and root dry weight showed that use of screened bacteria increased the numerical value and improved the environment of soybean rhizosphere. It was considered that the isolated bacteria from wild soybean rhizosphere could have biological control efficacy against pathogens of soybean root rot.

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Last Update: 2014-10-21