|Table of Contents|

PUT BT GENE TRANS FORMATION INTO SMATIC VIA MICROPROJECTILE BOMBARDMENT(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
140-143
Research Field:
Publishing date:

Info

Title:
PUT BT GENE TRANS FORMATION INTO SMATIC VIA MICROPROJECTILE BOMBARDMENT
Author(s):
WANG Xiao-chun1; WANG Gang2; JI Jing2; WANG Ping3
1.Department of Agriculture Sciences of Hebei North University， Xuanhua 075131；
2.Tianjin University of Agricultural and Biological Engineering，Tianjin 300072；
3.Huahai Institute of Technology, Marine School,Lianyungang,222005
Keywords:
Soybean; Somatic embryos; Bt gene; Genetic transformation
PACS:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.02.004
Abstract:
The Somatic embryo of soybean Hefeng 25 were transformed by microprojectile bombardment.The resistant plants to kanamycin were obtained,two positive plants were obtained among them by PCR,and the positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. The results demonstrated that the target Bt gene had been transformed into the soybean genome.

References:

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Last Update: 2014-10-21