|Table of Contents|

CONSTRUCTION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED CDNA LIBRARY FROM SOYBEANS INDUCED BY DARKNESS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年02期
Page:
134-139
Research Field:
Publishing date:

Info

Title:
CONSTRUCTION AND ANALYSIS OF DIFFERENTIALLY EXPRESSED CDNA LIBRARY FROM SOYBEANS INDUCED BY DARKNESS
Author(s):
ZHAO Lin1;  LUO Qiu-lan1;  YANG Chun-liang2; HAN Ying-peng1; LI Wen-bin1
1.Soybean Research Institute, Key Laboratory of Soybean Biology of Chinese Education Ministry),Northeast Agriculture University, Harbin,150030,
2.The Basic Medical Science college, Harbin Medical University, Harbin,150081
Keywords:
Soybean; SSH; Short day; Photoperiod; Senescence
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.02.003
Abstract:
Soybean was a shortday plant, and its growth and development was very sensitive to photoperiod response which seriously limited the adaptation of soybean variety and improvement in yield of per unit area. The aim of this study reported in this paper was to break the limitation in soybean growth areas, determining what transcripts were increased in abundance in short day treated plants compared to long day treated plants during the photoperiod inductive flowering and senescent process on the cellular and molecular level, and further to discover the complicated mechanisms of flowering induction and senescence in the SD soybean leaves perceiving daylength. A cDNA subtractive library enriched for mRNAs encoding ESTs that increased in abundance during short days was constructed by suppression subtractive hybridization (SSH) from leaf tissues of soybean cultivar Dongnong L13′, a photoperiod sensitive cultivar，and 738 clones were selected from the library. A total of 148 clones were obtained by Reverse Northern blotting and these were sequenced that encoded 76 unique ESTs. The ESTs were annotated by similarity to orthologs and paralogs detected with Blastn and Blastx. The proteins predicted to be encoded were inferred to be involved in diverse physiological and biochemistrical functions such as transcription, signal transduction, programmed cell death, protein, macromolecule degradation, detoxification and defense, cell wall modification. This research established foundation to reveal the mechanisms of short day inducing flowering and accelerating senescence.

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Last Update: 2014-10-21