|Table of Contents|

DETECTION OF GENETICALLY MODIFIED COMPONENTS IN PRODUCTS DERIVED FROM SOYBEAN AND MAIZE(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
84-88
Research Field:
Publishing date:

Info

Title:
DETECTION OF GENETICALLY MODIFIED COMPONENTS IN PRODUCTS DERIVED FROM SOYBEAN AND MAIZE
Author(s):
WU Jia-lin; ZHANG Jing-ping; HU Lin; NIU Wei-min; YOU Feng-xing
Wuxi Center for Disease Control and Prevention，Wuxi 214002
Keywords:
Soybean; Maize; Processed products; Qualitative PCR; Genetically modified Components
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.019
Abstract:
The purpose of this study was to establish detection method on genetically modified components in products derived from soybean and maize using PCR technology. The presence of processed products were investigated by PCR detection of CaMV 35S promoter， and the presence of Round up Ready Soybean (RRS)， Bt176 Maximaizer in products derived from soybean and maize were further determined to detect their specific DNA fragments respectively by PCR. One bean cake sample and one bean curd sample were verified a 498bp RRS specific DNA fragment by PCR detection， Bt176 Maximaizer specific PCR products were obtained with the corn grain DNA samples used as PCR templates.The research also succeeded in settling 0.1% precision degree of genetically modified organisms which ensured its stable property. The results showed that PCR analysis for the transgene was feasible and reliable. The PCR analysis of transgenic components could be widely used in various transgenic plants and their products.

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Last Update: 2014-10-21