|Table of Contents|

COMPARATIVE STUDIES ON EXTRACTION OF GENOME DNA FROM SOYBEAN LEAF(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
60-65
Research Field:
Publishing date:

Info

Title:
COMPARATIVE STUDIES ON EXTRACTION OF GENOME DNA FROM SOYBEAN LEAF
Author(s):
ZHANG Wei; XIE Fu-ti; CAO Ping; GUO Yu-hua; SONG Xian-jun
College of Agronomy， Shenyang Agricultural University， Shenyang 110161
Keywords:
DNA extraction;  SSR;  CTAB;  SDS
PACS:
S565.103
DOI:
10.3969/j.issn.1000-9841.2007.01.014
Abstract:
Template DNA quality directly affects the effectiveness of PCR amplification. To investigate the optimum DNA extraction method from soybean leaf， the influencing factors such as extraction buffer， buffer concentration， extraction times， and extraction steps were analyzed. The results indicated that the alkali extraction method on leaf was faster than but inferior to the other two methods. Both CTAB and SDS method could produce high quality DNA. When the DNA quality was compared under different buffer concentration ranging from 1% to 4%， it was found the quality of DNA could meet need of SSR analysis except that the DNA degraded a little under 4% CTAB. The concentration of DNA increased as the extraction times decreased， while the purity of DNA descended. The quality of DNA could meet SSR requirement even extracted once by chloroform/isoamyl. Under different extraction steps， we extracted DNA 4 times continuously， or separated out DNA after extracting twice， then dissolved it and extracted twice more， compared to the former， the later had a bigger ratio of A260/A280， but a suitable ratio of A260/A230.

References:

［1］周思君.小样品大批量大豆模板DNA快速分离［J］.大豆科学，1999，18（4）：318-321.

［2］王灏， 王道杰， 谭小力，等.用于RAPD分析的油菜总DNA 的快速提取［J］.西北农业学报，2001，10(3)：32- 34.
［3］戴思兰，陈俊愉，高荣孚，等.DNA提纯方法对9种菊属植物RAPD的影响［J］.园艺学报，1996，23 （2）：169-313.
［4］杜何为，黄敏，张祖新.玉米DNA的小量快速提取［J］.玉米科学，2004，12（2）：114-115.
［5］张永明，孙彩云，梁承邺.一步法提取植物DNA 用于大规模RAPD分析［J］.遗传，2000，22(2)：106.
［6］郭景伦，赵久然，辛景树，等.玉米单株幼芽DNA快速提取新方法［J］.华北农学报，2005，20（1）：38-40.
［7］Wang Shu，R E Knox，R M DePauw，J et al.A Simple DNA Preparation Method for PCR Amplifications in Marker.Assisted Selection of Wheat［J］.Agricultural Sciences in China，2005，4(7)：481-485.
［8］王晓丹，吕慧颖，张敬，等.以PCR为目的的大豆叶片DNA提取方法的比较研究［J］.分子植物育种，2004 2（6）：891-894.
［9］汪小全，皱育苹，张大明，等.RAPD应用于遗传多样性和系统学研究中的问题［J］.植物学报，1996，38（12）：954-962.
［10］卢阳江，郑康乐.提取水稻DNA的一种简易方法［J］.中国水稻科学，1992，6（1）：47-48.
［11］郑康乐，钱慧荣，庄杰云，等.应用DNA标记定位水稻抗稻瘟病基因［J］.植物病理学报，1995，25（4）：307-313.

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Last Update: 2014-10-21