|Table of Contents|

TRANSFOMING AGROBACTERIUM INTO SOYBEAN BY MEANS OF POLLEN TUBE PATHWAY INDUCED BY CaCl2(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
55-59
Research Field:
Publishing date:

Info

Title:
TRANSFOMING AGROBACTERIUM INTO SOYBEAN BY MEANS OF POLLEN TUBE PATHWAY INDUCED BY CaCl2
Author(s):
LI Hui; WU Tian-long
Agriculture & Biology College， Shanghai Jiaotong University， Shanghai, 201101
Keywords:
Pollen tube pathway;  CaCl2;  Soybean; pCAMBIA3301;  Agrobacterium
PACS:
S565.103.52
DOI:
10.3969/j.issn.1000-9841.2007.01.013
Abstract:
In order to improve transgene efficiency of pollen tube pathway， and study the possibility of direct transformation of Agrobacterium， pCAMBIA3301 at 3 concentration levels of CaCl2 was transformed into Shanghai Bendiqing Soybean receptors by means of pollen tube pathway， meanwhile， 3 different varieties of Agrobacterium GV3101、LBA4404、EHA105 were introduced into soybean as another treatment. When pCAMBIA3301 was served as foreign gene transformed by pollen tube pathway， an average seed-set percentage of 36.83% and an average seedling percentage of 60.89% were achieved. An average seedling survival percentage was 8.24% when 0.2% Besta was conducted with T1 plants. When AgrobacteriumGV3101，LBA4404，EHA105 were served as foreign gene transformed by pollen tube pathway， an average seed-set percentage of 15.92% and an average seedling percentage of 20.40% were achieved. An average seedling survival percentage was 7.79% when 0.2% Besta was conducted with T1 plants. Leaf genome DNA from survived T1 plant of different treatments were analyzed by PCR， 20 positive plants were obtained with pCAMBIA3301 as foreign gene， 3 concentration levels of CaCl2 gained 0.56%， 1.64%，1.40% efficiency respectively， 2 of which were about 3 fold higher than that of traditional way. 5 positive plants were obtained which 3 different varieties of Agrobacterium were directly introduced into soybean， gained 0.17%， 0.33%， 0.29% efficiency respectively.

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Last Update: 2014-10-21