|Table of Contents|

COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
51-54
Research Field:
Publishing date:

Info

Title:
COMPARISON STUDY OF THREE AFFINITY CHROMATOGRAPHY SYSTEMS ON SOYBEAN AGGLUTININ PURIFICATION
Author(s):
WANG Li-min; QIN Gui-xin; LIU Lin-na; ZHANG Liang-liang; ZHANG Ling-ling
College of Animal Science and Technology，Jilin Agricultural University，Changchu 130118
Keywords:
Affinity chromatography; Purification; Soybean agglutinin
PACS:
S565.101
DOI:
10.3969/j.issn.1000-9841.2007.01.012
Abstract:
Purification of soybean agglutinin with three affinity chromatography systems， GalNac- epoxy-Sepharose 6B，Gal-CH-Sepharose 4B and guar were compared.The results showed the differences of the prodution rate and absorbing capcities among the three systems were significant， 147±15，110±12， 128±16mg/100g raw soybean and 12.2±1.4，7.5±1.3， 2.1±1.6 mg/mL gel separately.This result indicated that the best way to purify soybean agglutinin was GalNac- epoxy-Sepharose 6B.

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Last Update: 2014-10-21