|Table of Contents|

IN SILICO CLONING AND EVOLUTION ANALYSIS OF DEHYDROASCORBATE REDUCTASE cDNA FROM GLYCINE MAX(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
45-50
Research Field:
Publishing date:

Info

Title:
IN SILICO CLONING AND EVOLUTION ANALYSIS OF DEHYDROASCORBATE REDUCTASE cDNA FROM GLYCINE MAX
Author(s):
LI Xiao-xiao; LI Rui; LI Ya-xuan; CAI Min-hua; HU Ying-kao
College of Life Science，Capital Normal University，Beijing 100037
Keywords:
Glycine max;  In silico cloning;  Dehydroascorbate reductase;  RT-PCR;  EST
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.011
Abstract:
Using bioinformatics strategy and validating by experiment， a novel dehydroascorbate reductase gene(DHAR) of Glycine max was cloned and identified which was being blasted by search of Glycine max EST database with homologous gene cDNA of Arabidopsis thaliana. This sequence was confirmed by RT-PCR， molecular cloning and sequencing. The full length Glycine max DHAR gene cDNA of 955bp(GenBank accession，DQ006810) encoded dehydroascorbate reductase (DHAR) of 259 amino acid and contained a complete ORF of 777bp. Compared with several plants， we found the gene was almost conserved. The results revealed that it was a convenient technique for cloning novel gene by searching EST database with homologous gene of model plants such as the Arabidopsis thaliana.

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Last Update: 2014-10-21