|Table of Contents|

OPTIMIZATION OF SSR DETECTION SYSTEM IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
36-40
Research Field:
Publishing date:

Info

Title:
OPTIMIZATION OF SSR DETECTION SYSTEM IN SOYBEAN
Author(s):
SONG Xian-jun; XIE Fu-ti; ZHANG Wei; CAO Ping; GUO Yu-hua
College of Agronomy， Shenyang Agricultural University， Shenyang 110161
Keywords:
Soybean; SSR; Detection system; Optimization
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.009
Abstract:
Effects of major components of PCR amplification on soybean SSR detection results were studied. A SSR detection system suitable for soybean was established with improved PCR reaction system， PCR program and silver staining method. The optimized PCR amplification system was 10×Buffer 1.00μL， 2.00 ng/μL template DNA， 0.15 μmol/L SSR primer， 150.00μmol/L dNTPs， 0.50 U Taq enzyme， 2.00 mmoL/L Mg2+， adding ddH2O to terminal volume 10.00 μL. The optimized PCR cycles started at 94℃(3min)，followed by 35 cycles of 25s at 94℃，25s at 47℃ and 30s at 72℃， and then extended 5min at 72℃.The optimized soybean SSR detection system was proved more economic and useful.

References:

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Last Update: 2014-10-21