|Table of Contents|

TWO-DIMENSIONAL ELECTROPHORESIS OF MIC-ROSOMAL MEMBRANE BOUND PROTEINS IN SOYBEAN COTYLEDONS(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
11-15
Research Field:
Publishing date:

Info

Title:
TWO-DIMENSIONAL ELECTROPHORESIS OF MIC-ROSOMAL MEMBRANE BOUND PROTEINS IN SOYBEAN COTYLEDONS
Author(s):
QI Yong-yan1; LIU Fu-hai2; Arnaud Bovy3; Jan Cordewener3; Froukje Vander Wal3
1.Bee Research Institute of Chinese Academy of Agricultural Sciences， Beijing， 100093；
2. Tianbaokang High-tech Co. Ltd., Beijing, 100097;
3. Plant Research International， P.O. Box 16， 6700 AA， Wageningen， the Netherlands
Keywords:
Two-dimensional electrophoresis; Glycine max (L.) Merri;  Cotyledons;  Microsomal membrane bound proteins
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.004
Abstract:
Soybean cotyledons induced by silver nitrate were used to investigate the work of two-dimensional electrophoresis (2DE) on the separation of microsomal membrane bound proteins. The following influencing factors on the solubilization of membrane bound proteins and 2DE images were discussed， such as the composing of rehydration solution， sample amount， focusing total voltage hours and different range of immobilized pH gradients (IPG) strips. It was shown that the samples rehydrated in solution making of urea， thiourea， tributyl phosphine and (3-(4-hepty) phenyl 3-hydroxy propyl)(dimethylammonio propane sulfonate)(C-7BZO) could be seen much better 2DE images than that of the other tested rehydration solutions. At the same time， the other running conditions for 70×3×0.5mm IPG strips were optimized as that the sample amount was 80μg and the focusing total voltage hour was around 20000. Much more clear spots were received by the usage of narrow-range IPG strips than that of wide-range IPG strips. Thirteen distinguish spots were found only in the induced samples and not in the control samples， whose isoelectric points (pI) were 4.0~8.0.

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Last Update: 2014-10-21