|Table of Contents|

CLONING， SEQUENCE ANALYSIS AND ANTIGEN EPITOPE PREDICTION OF PANALLERGEN PROFILIN IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2007年01期
Page:
1-5
Research Field:
Publishing date:

Info

Title:
CLONING， SEQUENCE ANALYSIS AND ANTIGEN EPITOPE PREDICTION OF PANALLERGEN PROFILIN IN SOYBEAN
Author(s):
WU Kai-wei; YU Xiao; LIU Zhi-gang; SONG Juan-juan; ZHANG Hong-yun
College of Life Science，Shenzhen University，Shenzhen，518060
Keywords:
Glycine max; Panallergen; Profilin; Gene cloning
PACS:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.01.002
Abstract:
Bioinformatic method was used for the comparative analysis of numerous homologous plant food panallergen and pollen panallergen sequences. Conservative domains among the sequences were determined for degenerate primer designing. The defined RT-1PCR conditions were adopted to clone the full-length gene of profilin from soybean and a novel full-length cDNA clone (396bp， pI 5.21， MW 14，1kD) was obtained. Sequence analysis showed that this gene shared high identities (＞75%) with panallergen profilin from some plant food and pollen. The deduced protein was therefore regarded as panallergen and named as Gly m 3.02 (EMBL/GenBank database entry No. DQ784852). This novel panallergen profilin gene from soybean will be a base for the expression of allergen proteins and analysis of epitope applied in further studies and clinical treatment.

References:

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Last Update: 2014-10-21