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CLONINGOF SOYBEAN FERRITIN GENE AND CONSTRUCTIONOF ITS PLANT EXPRESSION VECTOR(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年04期
Page:
454-457
Research Field:
Publishing date:

Info

Title:
CLONINGOF SOYBEAN FERRITIN GENE AND CONSTRUCTIONOF ITS PLANT EXPRESSION VECTOR
Author(s):
Wang Yongbin 1 　Guo Changhong 1 　Guo Donglin 1 　Cai Qiuyi 1 　Zhang Shuang 1 　Li Jilin 1 　Lin Hong 2
(1. Department of Biology , Harbin Normal University , Harbin, 1500802. Mudanjiang Pharmaceutical Factory , Mudanjiang 157013)
Keywords:
soybean ferritin ; cDNA ; cloning ; plant expression vector
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.04.0454
Abstract:
　A 750bp fragment was amplified by RT-PCR from soybean. The PCR products were cloned intopBlueskript SK +vector and named pFer after its sequences was confirmed. The soybean ferritin gene wassubcloned into plant expression vector pBI121 and named PBI121Fer. Then the recombination plasmidwere introduced into Agrobacterium EHA105. This work provides a foundation for transferring ferritingene into plant by Agrobacterium mediated transformation.

References:

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Last Update: 2016-12-16