|Table of Contents|

GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T- DNA BINARY VECTOR(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年04期
Page:
369-372
Research Field:
Publishing date:

Info

Title:
GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T- DNA BINARY VECTOR
Author(s):
Zhang Xiuchun 1 　 Peng Ming 1 　 Wu Kunxin 1 　 Guo Liqiong 1; 2 　 Lin Junyang 　 Lin Junfang1; 2
(1. State Key Laboratory of Tropical Crop Biotechnology , Chinese Academy of Tropical AgriculturalSciences, Haikou 571101 ;2 Department of Bioengineering , Collegeof Food Sciences , South ChinaAgricultural University , Guangzhou 510640;3 Putian City’s Institute ofAgricultural Sciences , Putian 351100)
Keywords:
Double T - DNA ; Marker-free ; Soybean
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.04.0369
Abstract:
T ransgenic crops have developed in commercialized scale in some countries, but their biosafetyhave attracted public concerns in the world at the same time. Therefore , it is becoming more and more im-portant to eliminate the selective genes because of their negative functions, such as antibiotics resistanceand herbicide resistance. 161 T 1 transgenic soybean of pudou 8008 containing Δ6- fatty acid desaturasegene and bar selection gene , which were obtained by biolistic particle co-transformation, were analyzed byPCR and southern blot in this study , and 9 plants only with the target gene were identiffed finally. Moreo-ver , leaf painting of herbicide was applied to confirm the marker free transgenic plants. It could be more ef-ficient to combine leaf painting and PCR, Southern blot to select bar free transgenic soybean.

References:

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Last Update: 2016-12-16