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2- DE ANALYSIS OF PROTEINS DURING SEED GERMINATION IN SOYBEAN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年04期
Page:
355-359
Research Field:
Publishing date:

Info

Title:
2- DE ANALYSIS OF PROTEINS DURING SEED GERMINATION IN SOYBEAN
Author(s):
Xu Xiaoyan1　 Zheng Rui1; 2　 Li Chunmei1　 Yu Deyue1*
(1. Nanjing Agricultural University , Soybean Research Institute , National Center f or SoybeanImprovement , and National Key Laboratory of Crop Genetics and Germplasm Enhancement ,Nanj ing 210095;2. College of Li fe Science , Ningxia University , Yinchuan 750021)
Keywords:
Soybean ; Two-dimensional gel electrophoresis (2- DE); Germination
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.04.0355
Abstract:
Two-dimensional gel electrophoresis (2 - DE) was utilized to analyze proteins with differentialexpressions during soybean seed germination. It was found that 341 and 376 protein spots were present indry mature seeds and germinated seeds in 2- DE maps, stained by coomassie brilliant blue and analyzed byPDQuest image software. The 2 - DE map of germinated seed was very similar to that of dry mature seed,suggesting that most of soybean seed storage proteins weren’t mobilized during seed germination. And 21up-regulated proteins and 9 down-regulated proteins were found during seed germination. The further i-dentification for these protein spots would help to understand the physiological and molecular mechanismsof soybean seed germination.

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Last Update: 2016-12-16