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THE STUDYON THE SCREENINGOF TRANSGENE SOYBEAN BY WAYOF POLLEN-TUBE USING KANAMYCIN(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年03期
Page:
275-278
Research Field:
Publishing date:

Info

Title:
THE STUDYON THE SCREENINGOF TRANSGENE SOYBEAN BY WAYOF POLLEN-TUBE USING KANAMYCIN
Author(s):
Xu Pengfei1　Zhang Shuzhen1　Wu Junjiang2　Han Yingpeng1　Zhang Dayong1Li Wenbin1　Yang Chuanping3
(1.Soybean Research Institute of Northeast Agricultural University , Harbin 150030;2. Heilongjiang Acadeng of Agricultural Sciences , Harbin 150086;3. North-east Forestry University , Harbin 150040)
Keywords:
Soybean ; Pollen-tube way ; Kanamycin
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.03.0275
Abstract:
Kanamycin was used to screen T 0 seeds of transgene Dongnong 46 by way of pollen-tube. The re-sults showed that 400ppm kanamycin could inhibit growth of non-transgene plants , and positive plantscould be gotten using Gus and PCR detection. It was proved that this method wa economic and effective inscreening the transgene plants.

References:

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Memo

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Last Update: 2016-12-15