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APLLICATION OF GENE SPLICING BY OVERLAP EXTENSIONON Glycine max PHENYLALANINE AMMONIA LYASE(PAL)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年03期
Page:
228-233
Research Field:
Publishing date:

Info

Title:
APLLICATION OF GENE SPLICING BY OVERLAP EXTENSIONON Glycine max PHENYLALANINE AMMONIA LYASE(PAL)
Author(s):
Song Jian 1 Ma Huiqin 2 　 Chen Shangwu 1
(1.College of Food Science &Nutritional Engineering ,Bejing 100083 ;2.Department of Pomology ,China Agricultural University ,Beijing 100094)
Keywords:
SOE; cDNA ; Phenylalanine ammonia lyase (PAL)
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.03.0228
Abstract:
Abstract　 T he two exons of Glycine max phenylalanine ammonia lyase (PAL) were recombined togetherby gene splicing by overlap extension (gene SOEing). Results showed that gene SOEing was a successfulspecific PCR technique for gene recombination. It was the improvement of cloning cDNA.

References:

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Last Update: 2016-12-15