|Table of Contents|

IDENTIFICATION AND SEQUENCE ANALYSIS OF COAT PROTEINGENE OF FIVE SOYBEAN MOSAIC VIRUS ISOLATES(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2006年03期
Page:
218-222
Research Field:
Publishing date:

Info

Title:
IDENTIFICATION AND SEQUENCE ANALYSIS OF COAT PROTEINGENE OF FIVE SOYBEAN MOSAIC VIRUS ISOLATES
Author(s):
Guo Dongquan　 Zhi Haijian 　 Wang Yanwei 　 Li Haichao 　 Yang Hua 　 Li KaiBai Li 　 Gai Junyi
(Soybean Research Institute of Nanj ing Agricultural University , National Center f or SoybeanImprovement Key Laboratory of Gop Genetics and Germplasm Enhancement , Nanjing 210095)
Keywords:
Soybean mosaic virus; Coat protein gene ; Sequence analysis
PACS:
-
DOI:
10.11861/j.issn.1000-9841.2006.03.0218
Abstract:
5 SMV isolates were obtained by using biological purification and serology (DAS-ELISA). Thecoat protein gene regions were amplified by reverse transcription polymerase chain reaction (RT-PCT). Se-quences analysis showed that CP gene of the each SMV isolates had 795 nucleotides in length , encoding265 amino acids. Comparison of the sequences of CP genes between different isolates showed they shared91. 1%～ 97. 1%nucleotide acids identities and 98. 1%～ 99. 2 %amino acidsidentities with each other. Theresults also revealed that there was significant correlation between CP genes of SMV and symptom reactionof SMV on differential Hosts

References:

1　 Chao E K , Goodman R M . Strains of soybean mosaic virus classi-fication based on virulence in resistant soybean cultivars, Phyto-pathology. 1979, 69 (5):467 - 470.

2　 濮祖芹, 曹琪, 房德纯. 大豆花叶病毒的株系鉴定[ J] . 植物保护学报, 1982, 9(1):15 - 19.
3　 陈永萱, 薛宝娣, 胡蕴珠, 等. 大豆花叶病毒(SMV)两个新株系的鉴定[ J] . 植物保护学报, 1986, 13(4):221- 226.
4　 吕文清, 张明厚, 魏培文. 东北三省大豆花叶病毒(SMV)株系的种类与分布[ J] . 植物病理学报, 1985, 15(4):225 - 228.
5　 张明厚, 魏培文, 张春泉. 我国东北五省市SMV 对大豆主栽品种的毒力测定[ J] . 植物病理学报, 1998, 28(3):237 - 242.
6　 罗瑞梧, 尚佑芬, 杨崇良. 山东省大豆花叶病毒株系鉴定[ J] . 山东农业科学, 1990,(5):16 - 19.
7　 尚佑芬, 赵玖华, 杨崇良, 等. 黄淮地区大豆花叶病毒株系鉴定[ J] . 山东农业科学, 1997,(6):24 - 27.
8　 王修强, 盖钧镒, 濮祖芹. 黄淮和长江中下游地区大豆花叶病毒株系鉴定与分布[ J] . 大豆科学, 2003, 22(2)102 - 107.
9　 Jayaram C H , Hill J H, Allen M W. Nucleotide sequence of thecoat protein genes of two aphid-transmissible strains of soybeanmosaic virus[ J] . Journal of General virology. 1991, 72:1001 -1003.
10 Eggenberger A L , Stark D M , Beachy R N. The nucleotide se-quence of soybean mosaic virus coat protein-colding region and itsexpression in E. Coli, Agrobacterium tumefaciens and tobaccocallus[ J] . Journal of General Virology , 1989, 70:1853 - 1860.
11 刘俊君, 彭学贤. 大豆花叶病毒外壳蛋白基因的克隆及其在大肠杆菌中的表达[ J] . 生物工程学报, 1993, 9(3):198 - 203.
12 储瑞银, 冷晓红, 鲍一明. 应用聚合酶链式反应扩增大豆花叶病毒外壳蛋白基因及其序列分析[ J]. 植物学报, 1992, 34 (7):523 -528.
13 董宏平, 程宁辉, 濮祖芹. 大豆花叶病毒Sc 株系外壳蛋白基因的部分序列分析[ J] . 南京师大学报, 1998, 21 (3):67 - 71.
14 黎昊雁, 陈炯, 陈剑平. 大豆花叶病毒杭州分离物基因组全序列测定及其结构分析[ J]. 科技通报, 2003, 3(2):90 - 93.
15 Atrey a P L, A tueya C D, Pirone T P. Amino acid aubstitutions inthe coat protein result in loss of insect transmissibility of an plantvirus[ J] . Proc. Natl. Acad. Sci. USA , 1991, 88:7887 - 7891.

Memo

Memo:
-
Last Update: 2016-12-15